let-7/miR-98家族对IL-6的调节作用

目的探索微小RNA对IL-6的调节作用。方法通过生物信息学预测发现let-7/miR-98家族可以调控IL-6基因,采用聚合酶链反应(RT-PCR)和酶联免疫法检测其对IL-6基因表达的影响,采用定点突变和双荧光报告基因系统来验证let-7/miR-98家族对IL-6基因的直接调节作用。结果 miR-98可以抑制IL-6蛋白的表达,miR-98对IL-6 mRNA也有抑制作用,let-7/miR-98可直接调节IL-6基因3’UTR。结论 let-7/miR-98家族可以直接调节IL-6基因的表达,提示let-7/miR-98家族可能成为干预IL-6参与的相关疾病的一种新药物。

Regulatory role of the let-7/miR-98 family in IL-6 expression

Objective To investigate the regulatory role of microRNA in expression of interleukin-6(IL-6).Methods The let-7/miR-98 family was predicted by bioinformatics to regulate IL-6.Real-time PCR and ELISA were used to determine IL-6 mRNA and protein expression levels,respectively.Site-directed gene mutagenesis and reporter gene assay were performed to identify the direct regulatory effect of let-7/miR-98 on IL-6 3′UTR.Results miR-98 can inhibit IL-6 expression at both protein and mRNA levels.Furthermore,let-7/miR-98 can directly target IL-6 3′UTR.Conclusion Let-7/miR-98 down-regulates IL-6 expression.The findings indicate that let-7/miR-98 may provide a novel strategy for therapeutic intervention of IL-6-related diseases.