无梗五加果中总香豆素和6,7-二甲氧基香豆素的含量测定

目的测定无梗五加果中总香豆素及6,7二甲氧基香豆素的含量。方法以东莨菪内酯为对照品,采用分光光度法,于348 nm处测定总香豆素含量。采用HPLC法测定6,7二甲氧基香豆素含量。色谱柱为Hypersil BDS C18(4.6 mm×250 mm,5μm),流动相为甲醇磷酸水溶液(pH3.0)乙腈四氢呋喃(体积比为15∶77∶6∶2),检测波长为340 nm。结果总香豆素质量浓度在3.44~12.9 mg.L-1(r=0.999 2)内、6,7二甲氧基香豆素质量浓度在1~40 mg.L-1(r=0.999 9)内线性关系良好;平均回收率分别为100.6%、97.7%;总香豆素和6,7二甲氧基香豆素的质量百分含量分别为0.12%、0.0037%。结论方法准确、可靠,可为无梗五加果的质量控制提供参考依据。

Determination of the contents of total coumarin and 6,7-dimethoxycoumarin in fruit of Acanthopanax sessiliflorum(Rupr.et Maxim.)Seem

Objective To determine the contents of total coumarin and 6,7-dimethoxycoumarin in fruit of Acanthopanax sessiliflorum(Rupr.et Maxim.)Seem.Methods The content of total coumarin was determined with ultraviolet spectrophotometry at(348 nm) using scopoletine as reference.The separation and quantitation of 6,7-dimethoxycoumarin was performed on a Hypersil BDS C_(18)((4.6 mm)×(250 mm),(5 μm))with mobile phase consisted of methanol-H_3PO_4(pH3.0)-acetonitrile-tetrahydrofuran(V∶V∶V∶V=15∶77∶6∶2)and detection at(340 nm).The flow rate was(0.8 mL)·min~(-1).Results The standard curve was linear in the range of 3.44-12.9 mg·L~(-1) for total coumarin(r=0.999 2)and 1-40 mg·L~(-1) for 6,7-dimethoxycoumarin((r=0.999 9)).The average recoveries of total coumarin and 6,7-dimethoxycoumarin were 100.6% and 97.7%,respectively.Conclusions The methods are simple,accurate with good reproducibility and can be used to assess the quality of fruit of Acanthopanax sessiliflorum(Rupr.et Maxim.)Seem.