植物内生真菌Fusariums sp.LC-1次级代谢产物的研究

 目的 研究植物内生真菌Fusariums sp.LC-1发酵液的乙酸乙酯萃取物和菌丝体的丙酮提取物中的化学成分,以期得到有活性的先导化合物。方法 采用硅胶柱色谱、凝胶柱色谱、高效液相色谱等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构。结果 从植物内生真菌Fusariums sp.LC-1发酵液的乙酸乙酯萃取物和菌丝体的丙酮提取物中共分离得到17个化合物,分别鉴定为：环(甘-缬)二肽（1）、环(甘-异亮)二肽（2）、白僵菌素（3）、麦角甾醇（4）、3β,5α,6β,9α-tetrahydroxyergosta-7,22-diene（5）、fusaric acid（6）、dehydrofusaric acid（7）、对羟基苯甲酸（8）、环(羟脯-亮)二肽（9）、环(甘-脯)二肽（10）、环(丙-苯丙)二肽（11）、环(亮-丙)二肽（12）、环(亮-缬)二肽（13）、环(苯丙-甘)二肽（14）、环(亮-甘)二肽（15）、尿嘧啶（16）、胸腺嘧啶（17）。结论 其中,化合物1、2为首次从天然产物中分离得到,化合物5、9、12、13、14为首次从镰刀菌属中分离得到。

Secondary Metabolites from Endophyte Fungus Fusariums sp. LC-1

OBJECTIVE To study the chemical constituents of the endophyte fungus Fusariums sp.LC-1. METHODS The compounds were isolated from the ethyl acetate extract and acetone extract by silica gel column chromatography,Sephadex LH-20 column chromatography and HPLC techniques. And their structures were identified by the physicochemical properties and spectral analysis. RESULTS Seventeen compounds were obtained from the static liquid culture of endophyte fungus Fusariums sp. LC-1. They were identified as cyclo(Gly-Val)(1),cyclo(Gly-Ile)(2),beauvericin(3),ergosterol(4),3β,5α,6β,9α-tetrahydroxyergosta-7,22-diene(5),fusaric acid(6),dehydrofusaric acid(7),p-hydroxybenzoic acid(8),cyclo(Hyp-Leu)(9),cyclo(Gly- Pro)(10),cyclo(Ala-Phe)(11),cyclo(Leu-Ala)(12),cyclo(Leu-Val)(13),cyclo(Phe-Gly)(14),cyclo-(Leu-Gly)(15),uracil(16)and thymine(17). CONCLUSION Compounds 1 and 2 are isolated from natural material for the first time. Compounds 5,9,12,13 and 14 are isolated from endophyte fungus Fusariums sp. for the first time.