针刺“人中”对失血性休克家兔心肌的组织化学观察

<正> 五十年代的生化研究就已证实,失血性休克时儿茶酚胺(CA)发生很大变化,起初由于交感-肾上腺髓质系统兴奋,使血中CA明显增加,但随着休克时间的延长,病情的严重,器官出现CA耗竭现象(包括心、肝等内脏),并认为CA耗竭对心脏交感神经活动不利。而以往的研究表明,针刺“人中”对失血性休克动物心脏功能有恢复作用。那么,这种恢复是否与CA以及交感神经的功能状态有关?故本文以组

THE EFFECTS OF ACUPUNCTURE AT "RENZHONG" POINT ON HEMORRHAGIC SHOCK'S MYOCARDIAL CATECHOLAMINE FLOURESCENCE. A Histochemical Study

32 healthy male rabbits were selected and divided at random into 4 gro- ups---hemorrhagic shock, acupuncture, sham operation and normal contol. After measuring the normal blood pressure and electrocardiograph, the ra- bbits in hemorrhagic and acupuncture group were bleeding 20ml/kg, 10ml/ min till the blood pressure dropped to 40-50 mmHg. Then acupuncture at "Renzhong" point was given 5 minuts per 20 minuts in acupuncture group. At the end of the experiment, ventricular myocardiums were excised and immediatly put into isopentane cooled by dryice-acetone (-70℃) and made cryostat section for histochemical observations of catecholamine flourescence ribonucleic acid and acid fuchsin-methyl green method. The result shows that the CA amount in hemorrhagic group(measured with Leitz MPV2 microflometry) is much lower than normal control and sham operation(with statistic significance), and it is much higher in acupu- ncture group than hemorrhagic group (with statistic significance), nearly the same as the normal level. So it was seen that the CA flourescent ne- rves in acupuncture group is much denser and brighter than the nerves in hemorrhagic group. Therefore it is considered that acupuncture can incre- ase the CA amount and regulate the sympathetic adrenergic nerves to make the heart working in a nearly normal state.