家兔心肺复苏后心肌肌钙蛋白T脱失的免疫组化研究

目的:观察家兔心肺复苏(CA-CPR)后心肌组织内心肌肌钙蛋白T(cTnT)的脱失情况,探讨心肌损伤的机制。方法:采用家兔缺氧型CA-CPR模型,于心肺复苏前后分时间点抽取动脉血,测血清cTnT值;自主循环恢复后4小时取心肌组织,应用免疫组化LSAB法显色观察cTnT表达的脱失情况。结果:自主循环恢复后血清cTnT明显升高。自主循环恢复后4小时心肌组织内cTnT明显脱失,图像分析显示心肺复苏后cTnT的脱失面积与正常家兔心肌之间存在显著性差异。结论:家兔心肺复苏后血清cTnT明显升高,心肌组织内cTnT的明显脱失,两者呈负相关。

Immunohistochemistry Study with Depletion of Cardiac Troponin T in the Myocardium of Cardiopulmonary Resuscitated Rabbits

Objective: To explore the mechanisms of myocardial injury, we investigated the loss of cardiac troponin T (cTnT) protein expression in the myocardium of cardiopulmonary resuscitated (CPR) rabbit models. Methods: Animal models of hypoxic cardiac arrest (CA) were made, and then the concentration of serous cTnT in arterial blood was tested before and after sucressful resuscitation. The animals were sacrificed 4 h after restoration of spantaneous civculation(ROSC) and myocardium were prepared for immunostain to detect the expression of cTnT. Results: (1) Serous cTnT ascended obviously after ROSC. (2) cTnT of myocardium showed obvious depletion 4 h after ROSC. The area of depletion was significantly qreater in experimental group than in the control group. Conclusion: After CA-CRP,Serous cTnT ascends and myocardial cTnT lost obviously. There is a negative correlation between them.