| HPLC法测定疏肝利胆胶囊中大黄素和大黄酚的含量 |
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| 引用本文： | 孙巍. HPLC法测定疏肝利胆胶囊中大黄素和大黄酚的含量[J]. 中国中医药杂志, 2008, 6(11): 1-4 |
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| 作者姓名： | 孙巍 |
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| 作者单位： | 吉林省吉林市第五医院,132001 |
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| 摘 要： | 目的：建立疏肝利胆胶囊的含量测定方法。方法：采用高效液相色谱法。色谱柱：SHIMADZU VP-ODS（250mm×4．6mm，5um），流动相：甲醇：0．1％磷酸溶液（80：20，V／V），流速：1．0ml·min^-1，检测渡长：254nm，柱温：30℃。结果：大黄素在10．13～250．8μg·ml^-1范围内线性关系良好（r=0．9996）；平均加样回收率为96．2％，RSD=1．2％（n=6）；大黄酚在10．00—250．0μg·ml^-1范围内线性关系良好（r=0．9998）；平均加样回收率为96．7％。KSD=1．3％（n=6）.结论：该方法简便易行，结果准确可靠，可适用于连翘败毒丸的质量控制。
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| 关 键 词： | 高效液相色谱法 疏肝利胆胶囊 大黄素 大黄酚 含量测定 |
Determination the content of Emodin and Chrysophanol in Shugan lidan Capsules by HPLC |
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| Sun Wei. Determination the content of Emodin and Chrysophanol in Shugan lidan Capsules by HPLC[J]. , 2008, 6(11): 1-4 |
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| Authors: | Sun Wei |
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| Affiliation: | Sun Wei (Jilin cidy medical Hospital No 5 Jilin Jilin 132001) |
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| Abstract: | objective To establish a HPLC method for determination the content of emodin and chysophanol in. Shugan lidan Capsules. Method：The shimadzu VPODS C18 （250mm× 4.6mm, 5um） was used, the mobile phase was methanol-0.1% phosphoric acid solution（80：20）,the column temperature was 30℃ ,the detection wavelength was 254 nm. the flow rate was 1.0ml · min^-1 .Results：The linear ranges of emodin and chrysophanol were at 10.13-250.8μg·ml^-1 （r=0.9996） and 10.00-250.0μg ·ml^-1 （r=0.9998） respectively, the average recoveries（n=6） were 96.2% and 96.7% with RSD 1.2% and 1.3% respeclivery. Conclusion：The method is simple and accurate, it can be used for quality control of shugan lidan Capsules. |
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| Keywords: | HPLC Shugan lidan Capsules emodin chrysophanol determination |
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