HPLC法测定疏肝利胆胶囊中大黄素和大黄酚的含量 |
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引用本文: | 孙巍.HPLC法测定疏肝利胆胶囊中大黄素和大黄酚的含量[J].中国中医药杂志,2008,6(11):1-4. |
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作者姓名: | 孙巍 |
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作者单位: | 吉林省吉林市第五医院,132001 |
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摘 要: | 目的:建立疏肝利胆胶囊的含量测定方法。方法:采用高效液相色谱法。色谱柱:SHIMADZU VP-ODS(250mm×4.6mm,5um),流动相:甲醇:0.1%磷酸溶液(80:20,V/V),流速:1.0ml·min^-1,检测渡长:254nm,柱温:30℃。结果:大黄素在10.13~250.8μg·ml^-1范围内线性关系良好(r=0.9996);平均加样回收率为96.2%,RSD=1.2%(n=6);大黄酚在10.00—250.0μg·ml^-1范围内线性关系良好(r=0.9998);平均加样回收率为96.7%。KSD=1.3%(n=6).结论:该方法简便易行,结果准确可靠,可适用于连翘败毒丸的质量控制。
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关 键 词: | 高效液相色谱法 疏肝利胆胶囊 大黄素 大黄酚 含量测定 |
Determination the content of Emodin and Chrysophanol in Shugan lidan Capsules by HPLC |
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Authors: | Sun Wei |
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Institution: | Sun Wei (Jilin cidy medical Hospital No 5 Jilin Jilin 132001) |
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Abstract: | objective To establish a HPLC method for determination the content of emodin and chysophanol in. Shugan lidan Capsules. Method:The shimadzu VPODS C18 (250mm× 4.6mm, 5um) was used, the mobile phase was methanol-0.1% phosphoric acid solution(80:20),the column temperature was 30℃ ,the detection wavelength was 254 nm. the flow rate was 1.0ml · min^-1 .Results:The linear ranges of emodin and chrysophanol were at 10.13-250.8μg·ml^-1 (r=0.9996) and 10.00-250.0μg ·ml^-1 (r=0.9998) respectively, the average recoveries(n=6) were 96.2% and 96.7% with RSD 1.2% and 1.3% respeclivery. Conclusion:The method is simple and accurate, it can be used for quality control of shugan lidan Capsules. |
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Keywords: | HPLC Shugan lidan Capsules emodin chrysophanol determination |
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