FoxP3 mRNA 在胃癌患者CD4 + CD25 + 调节性T 细胞中的表达及其临床意义

 目的 探讨胃癌患者体内CD4⁺CD25⁺调节性T细胞的含量及FoxP₃ mRNA在Treg中的表达情况及其临床意义。方法 应用流式细胞术（FCM）测定20例胃癌患者外周血中Treg细胞含量,同时采用RT-PCR技术检测其FoxP₃ mRNA的表达情况,并与20例健康志愿者的外周血进行对比研究和统计学分析。结果 胃癌患者外周血中Treg细胞占CD4⁺T细胞总数的（12．76±0．46）％,显著高于健康对照组的（6．81±0．66）％,（P〈0．01）;FoxP₃ mRNA在胃癌患者外周血Treg细胞中呈现高表达（0．84±0．02）％,明显高于其在健康对照组中表达（0．76±0．03）％,（P〈O．05）。结论 胃癌患者外周血中Treg的数量较正常人明显增高,同时伴随有FoxP,mRNA高表达,提示FoxP₃ ,基因可能对Treg有重要的调节功能,从而影响胃癌病人的抗肿瘤自身免疫功能。

Clinical Significance of FoxP3 mRNA Expression in CD4 + CD25 + Regulatory T Cells Isolated from Patients with Gastric Cancer

Objective 　To investigate the content s of CD4 ⁺ CD25 ⁺ T cells and the expression of FoxP₃ mRNA in these Treg cells in peripheral blood of the patients with gast ric cancer and to characterize their clinical significance. Methods 　By using flow cytometry ,the levels of CD4 ⁺ CD25 ⁺ T cells in peripheral blood of 20 preoperative patient s with gastric cancer were determined. RT-PCR techniques were used to detect the expression of FoxP₃ mRNA in these Treg cells ,and the results were analyzed statistically in comparison with that of 20 healthy volunteers. Results 　The percentage of CD4 ⁺ CD25 ⁺ T cells in total CD4 ⁺ T cells isolated f rom the patient s with gastric cancer was (12. 76 ±0. 46) % ,in comparison ,it was (6. 81 ±0. 66) % in those f rom healthy volunteers. It was obviously different between Treg cells amount of gastric patients and that of volunteers ( P < 0. 01) . The FoxP₃ mRNA expression of Treg cells in the patients with gastric cancer (0. 84 ±0. 02) %〕was significantly higher than that in healthy volunteers 〔(0. 76 ±0. 03) % , ( P < 0. 05) . Conclusion 　The levels of CD4 ⁺ CD25 ⁺ T cells in peripheral blood and the FoxP₃ mRNA expression of Treg cells in gast ric cancer patient s were increasing significantly. FoxP₃ genes may play a pivotal role in regulating the function of Treg cells in self-tumor immunity of the patient s with gastric cancer.