电针促进帕金森小鼠黑质致密部突触形态可塑性的研究

目的探讨电针在促进帕金森小鼠黑质致密部突触可塑性中的作用.方法以l-甲基-4苯基-1,2,3,6四氢吡啶诱导的帕金森小鼠作为研究对象,电针"合谷"、"太冲"穴,每日1次,7次为l疗程,共3个疗程后,分别运用尼氏染色和电镜观察神经元损害的大体和超微结构.结果帕金森模型小鼠尼氏染色阳性细胞数目减少,电针后增加(P＜0.01);模型组小鼠黑质致密部神经元核膜不清,染色质积聚,线粒体肿胀,内质网扩张;电针组神经元胞浆丰满,内质网较多,线粒体正常;模型组突触数目、数密度、面密度、突触平均连接面积较空白组、空电组增加,电针后呈现进一步增加趋势.结论电针刺激具有一定程度的促进帕金森小鼠黑质致密部神经元突触形态可塑性发挥的作用.

Enhanced Synapstic Plasticity in Parkinson's Disease Mice Sunbstantla Nigra by Electroacupuncture Therapy

Objective To determine whether electroacupuncture (EA) treatment enhance synaptic morphologic plasticity in Parkinson's disease (PD) mice substantia nigra compacta(SNC). Male mice(C57BL/6J) were averagely and randomly divided into controlled group,controlled EA group, model group and EA group. PD model mice were induced by i.p. 1-methyl,4-phenyl-1,2,3,6 tetrahydropyridine. We take EA treatment with frequency from 2 to 100HZ,volt arrange from 2 to 4V,points on Hegu(LI4) and Taichong(LV3).The treatment was once daily. After 21 times treatments, we killed all mice and observed synapse plasticity in SNC by electron microscope. The positive cell of nissles stain in PD mouce increased after electroacupuncture; the number and the number density increased in substantial nigra compared with PD, the volume density also enlarged. Conclusion] EA has a trendency to enhance degenerative dopaminergic neuron plasticity which need to study in depth.