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Inhibition of latrunculin-A on dexamethasone-induced fibronectin production in cultured human trabecular meshwork cells |
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| Authors: | Yun Wang Juan Tan Ye-Hong Zhuo Cui-Lan Wang Xu-Yang Liu Su-Ping Cai |
| Affiliation: | Ophthalmic Laboratories & Department of Ophthalmology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China,Department of Second Affiliated hospital of Nanhua University, 421001 Hunan Province, China,State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou Province 510060, China,Department of Second Affiliated hospital of Nanhua University, 421001 Hunan Province, China,Ophthalmic Laboratories & Department of Ophthalmology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China and Ophthalmic Laboratories & Department of Ophthalmology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China |
| Abstract: | AIMTo determine the effects of a low dose latrunculin (LAT)-A on dexamethasone (Dex)-induced upregulation of extracellular matrix proteins fibronectin (FN) in cultured human trabecular meshwork (HTM) cells.METHODSHTM cells were cultured to confluent and incubated with 0.4µmol/L Dex and/or 0.05µmol/L LAT-A. FN expression in HTM cells was evaluated by Western blot and immunofluorescence microscopy.RESULTSDex up-regulated FN production in HTM cells, failed to do so when co-incubated with LAT-A. LAT-A decreased production of FN in cultured HTM cells.CONCLUSIONThis study indicated that LAT-A may modulate the expression of fibronectin in trabecular meshwork to achieve treatment for steroids and other types of glaucoma. It has an important prospect as an intraocular pressure- lowering drug. |
| Keywords: | latrunculin-A dexamethasone human trabecular meshwork cells fibronectin |
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