泌尿生殖系四种病原体感染的实验室诊断分析

目的　探讨泌尿系生殖系淋球菌、沙眼衣原体、解脲支原体、人型支原体四种病原体感染与性传播疾病的关系 ,为临床提供实验室诊断依据。方法　对临床拟诊为泌尿生殖系感染 ,具有典型尿路刺激症状 ,近期未服用抗生素的患者取尿 (阴 )道分泌物 ,淋球菌检测采用淋球菌选择性培养基进行培养 ;支原体用法国生物 -梅里埃公司生产的Mycoplas maIST支原体培养鉴定试剂进行支原体 解脲支原体 (UU) ,人型支原体 (MH) ]沙眼衣原体采用金标免疫斑点法 ,由英国OXOID公司生产的立明试剂进行培养和鉴定。结果　 1 0 2 1 9例泌尿系感染患者中总的病原体检出率为 1 7.52 % (1 790 /1 0 2 1 9) ;淋球菌感染率为 2 .79% (44/1 579) ;沙眼衣原体感染率为 7.0 4 % (2 0 8/2 955) ;解脲支原体感染率为 36 .78% (1 2 76/3469) ;人型支原体感染率为 1 1 .82 % (2 62 /2 2 1 6)。结论　开展淋球菌、支原体培养及沙眼衣原体的检测对临床确诊淋病、非淋菌性尿道炎有着极重要的作用。

Laboratory diagnosis of urinary and reproductive system infection caused by four kinds of pathogen

Objective To explore the relationship between four kinds of pathogen of urinary and reproductive system infection and sexual transmitted diseases,and to provide laboratory reference for clinical diagnosis.Methods The secretion specimens of urethra or vagina were collected from patients who were suspected to suffered with urinary and reproductive system infection.All patients had typical urinery irritative symptom and were not treated with antibiotic lately.Neisseria gonorrhoeae(NG),Ureaplasma urealyticam(UU) and Mycoplasma hominis (MH) were cultured and isolated respectively by its corresponding selective culture medium. Chlamydia trachomatis (CT) was qualitative analyzed by dot-inmmunogold method.Results There were 1790 patients who suffered with urinary and reproductive system infection caused by the four kinds of pathogen among 10219 cases,total postive rate was 17.52%(1790/10219);The postive rate of NG, UU, MH and CT were 2.79%(44/1579),7.04%(208/2955),36.78%(1276/3469),11.82%(282/2216),respectively.Conclusions The detection of NG, MH, UU and CT is very important to the clinical diagnosis of gonorrhea and non-gono. coccalurcthritis.