针刺“内关”穴对心肌梗塞模型大鼠心脏微血管ATP酶的影响

目的:研究针刺“内关”穴对心肌梗塞后缺血区微血管ATPase活性变化的干预效果。方法:采用Mg2 + ATPase光镜酶组织化学和Na+ K+ ATPase电镜酶细胞化学方法,动态显示微血管内皮细胞的ATPase活性。结果:结扎冠脉后,血管内皮细胞出现缺血缺氧性损伤,ATPase活性降低(P <0 .0 5、0 .0 1 ) ,以2d时损伤最重;1周以后ATPase活性开始恢复,3周时仍未恢复到正常水平(P <0 .0 5 )。针刺组ATPase活性损伤较模型组为轻(P <0 .0 5、0 .0 1 ) ,其ATPase活性损伤修复较快,3周时恢复到正常水平(P >0 .0 5 )。结论:针刺“内关”穴具有改善缺血区微血管内皮细胞ATPase活性的功能。

Effect of Acupuncture of Neiguan (PC 6) on ATPase of Cardiac Micrangium in Acute Myocardial Ischemia Rats

Objective: To observe the effect of Electroacupuncture (EA) of “Neiguan”(PC 6) on ATPase activity of capillaries in acute myocardial ischemia (AMI) rats. Methods: Sixty-six Wistar rats were randomized into normal (n=3), sham-operation (n=3), model and EA groups, and the last two groups were further divided into 2h, 2d, 1w, 3w subgroups respectively, with 6 cases being in each subgroup. AMI model was established by occlusion of the descending anterior branch (DAB) of the left coronary artery. EA (40 Hz, 4 V) was applied to “Neiguan”(PC 6) for 5 min, once daily till sampling (2h, 2d, 1w, 3w). The reaction products of Mg 2+ -ATPase and Na+-K+-ATPase of the left cardiac ventricle and apical region tissues (cut into sections, 10 μm in the thickness) were detected by using enzyme histochemical staining and enzyme cytochemical staining techniques and observed under electronic microscope, and the optic density (OD) or grey scale (GS) values of the reaction products of the tissue sections were detected with a multimedia image analysis system. Results: Comparison among different groups showed that the total area values of Mg 2+ -ATPase and Na+-K+-ATPase reaction products of the vascular endothelium in the myocardial tissue of model group from 2h to 3w were significantly lower than those of normal group (P<0.05～0.01), those of EA group from 2h to 3w were significantly higher than those of model group (P<0.05～0.01). Regarding the average OD/GS values, compared with normal group, in model group, GS of Mg 2+ -ATPase reaction products increased significantly but OD of Na+-K+-ATPase reaction products lowered markedly (P<0.05～0.01); compared with model group, in EA group, GS of Mg 2+ -ATPase reaction products at different time courses decreased significantly further, while OD of Na+-K+-ATPase reaction products increased remarkably at all the time courses except 2w (P<0.05～ 0.01). In regard to the number of capillaries, those of model group from 2h to 3w were considerably fewer than that of normal group (P<0.01); compared with model group, those of EA group from 2 h to 2w were considerably more (P<0.05～0.01). No significant differences were found between normal and sham-operation groups in these indexes. Microscopic observation displayed that after AMI, the state of myocardial fiber fracture, edema, degeneration, necrosis, and the vague outline of micrangium in model group was severer, while that in EA group was lighter. Conclusion: EA of Neiguan (PC 6) has effects of improving ATPase activity in endothelial cells of the ischemic myocardial micrangium and protecting anoxic myocardial cells.