丹酚酸A对成纤维细胞活力、增殖及胶原合成的影响

目的：探讨庆酚酸Ａ对成纤维细胞增殖及胶原合砀影响。方法：常规培养ＮＩＨ／３Ｔ３成纤维细胞，１０＾－４～１０＾－７ｍｏｌ／Ｌ丹酚酸Ａ温有亚单２２ｈ。〖＾３Ｈ〗－ＴｄＢ主法测定细胞增殖。〖＾３Ｈ〗－ｐｏｒｌｉｎｅ掺入法测定细胞活力，〖＾３Ｈ〗－ｐｒｏｌｉｎｅ掺入，胶原酶消化法细胞内外胶原生成率。结果：１０＾－１～１０＾－６ｍｏｌ／Ｌ彤酚酸Ａ抑制细胞增殖，除１０＾－４〖＾３Ｈ〗ｍｏｌ／Ｌ丹酚酸Ａ组细胞〖

Effect of Salvianolic Acid-A on NIH/3T3 Fibroblast Viability, Proliferation and Collagen Synthesis

Aim: To study effect of Salvianolic Acid-A (SA-A) on NIH/3T3 fibroblast functions such as viability, proliferation and collagen synthesis. Methods: NIH/3T3 fibroblasts were cultured with regular method, and incubated with 10-4-10-7mol/L SA-A for 22h. The cell viability was assayed by [3H]-proline incorporation, cell proliferation by [3H] -TdR incorporation, cell collagen synthesis rate was measured with [3H] -proline incorporation collagenase digestion. Results 10-4-10-6mol/L SA-A could inhibited fibroblast except 10-4 mol/1 SA-A which decreased cell viability, and 10-6mol/L had the best effect among three groups also 10-5-10-5mol/L SA-A inhibited intracellular collagen synthesis. 10-5mol/L showed a better effect. but both 10-5-10-6mol/L SA-A had no significant influence on extracelluar collagen secretion. Conclusion : SA-A could inhibited fibroblast proliferation and intracellular collagen synthesis, the best drug concentration perhaps was 10-6mol/L in vitro.