In vitro skin expansion: Wound healing assessment |
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| Authors: | Peter M Prim PhD Han Su Kim MD PhD Lindsey E Shapiro BS Jae Sung Lee MD PhD James H Kaan BS John D Jackson PhD James J Yoo MD PhD Anthony Atala MD Sang Jin Lee PhD |
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| Institution: | 1. Wake Forest School of Medicine, Wake Forest Institute for Regenerative Medicine, Winston‐Salem, North Carolina;2. Industrial and Systems Engineering, North Carolina State University, Raleigh, North Carolina;3. Department of Otorhinolaryngology, School of Medicine, Ewha Womans University, Seoul, South Korea;4. Department of Orthopedic, School of Medicine, Chung‐Ang University, Seoul, South Korea |
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| Abstract: | For treatments requiring split‐thickness skin grafts, it is preferable to mesh the grafts. This reduces the amount of excised skin and covers more wound area. The mesh technique, however, destroys surface continuity, which results in scarring. Strain‐based bioreactors, on the other hand, have successfully expanded split‐thickness skin grafts in vitro within a 7‐day period, increasing graft coverage. After in vitro expansion, the expanded skin grafts were tested in a porcine full‐thickness excisional wound model. Expanded graft take rate was 100%. Volumetric, histologic, and mechanical assessments indicated that expanded grafts were comparable to unexpanded grafts (positive control). While there was considerable variation in expansion (31% to ?3.1%), this technique has the potential to enhance the coverage area of skin grafts while reducing or eliminating scarring. |
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