箭根薯愈伤组织诱导及无性系的建立

目的 诱导箭根薯愈伤组织及建立无性系.方法 以箭根薯野生种为材料,采用不同激素组合对不同部位(根茎、叶片、叶柄)进行诱导,对比诱导效果,筛选出最佳部位及各个生长阶段的最佳激素组合.结果 外植体的消毒以0.1％ HgC12溶液处理8 min的效果最佳；诱导愈伤组织最佳外植体为叶柄；愈伤组织诱导的最佳培养基是MS +6-BA 2.0 mg/L+NAA 0.1mg/L+2,4-D 1.0 mg/L,在此培养基上使用叶柄诱导愈伤组织的诱导率达到100％;不定芽诱导分化的最适培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,分化率高达90％；生根诱导的最佳培养基为含1/2 MS+NAA 0.5 mg/L,生根率高达100％.结论 筛选出诱导箭根薯愈伤组织最佳诱导外植体及激素组合.

Callus induction and asexual line establishment of Tacca chantrieri

Objective To induce the callus and to establish the asexual line for Tacca chantrieri. Methods The wild T. chantrieri was used to investigate the induction effects on different hormone combinations and different explants (rhizomes, leaves, and leaf stalks), and the best explants and proper hormone combination for every growth period were established. Results The treatment with 0.1% HgCl₂ for 8 min was suitable to sterile the explants, the leaf stalk was the best material in callus induction among the three explants, and the best hormone combination for callus induction was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L + 2, 4-D 1.0 mg/L. The callus induction rate from leaf stalk was 100%, the suitable medium for differentiation on bud was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L, the differentiation rate was 90%, the best rooting medium was 1/2 MS + NAA 0.5 mg/L, and the rooting rate was 100%. Conclusion The best explants and hormone combination are screened out.