| 酸性染料比色测定皖贝总碱含量方法的考察 |
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| 引用本文： | 李成网,李清华,李矗,王珍. 酸性染料比色测定皖贝总碱含量方法的考察[J]. 现代中药研究与实践, 1999, 0(3) |
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| 作者姓名： | 李成网 李清华 李矗 王珍 |
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| 作者单位： | 安徽省医学科学研究所!合肥230061(李成网，李清华)，安徽省立医院!合肥230001(李矗)，安徽省医药科技实业公司!合肥230022(王珍) |
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| 摘 要： | 目的:建立酸性染料比色测定皖贝总碱含量的方法。方法:采用酸性染料比色法,对该方法的实验条件进行选择。结果:皖贝总碱在2.0～10.0μg/ml浓度范围内,选用pH5.0邻苯二甲酸氢钾缓冲液2ml和0.001mol/L溴麝香草酚兰溶液2ml,在410nm波长处,显色后70min内测定吸收度。结论:本法具有灵敏度高,结果准确,重复性好等特点。
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| 关 键 词： | 皖贝母 皖贝总碱 酸性染料比色法 |
Determination of Total Alkaloids in Wan beimu(Fritillaria anhuiensis) by Adid Dye Colorimetry |
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| Li Chengwang,Li Qinghua. Determination of Total Alkaloids in Wan beimu(Fritillaria anhuiensis) by Adid Dye Colorimetry[J]. Research and Practice on Chinese Medicines, 1999, 0(3) |
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| Authors: | Li Chengwang Li Qinghua |
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| Abstract: | VE:The method was reported for determination of total alkaloids in Wanbeimu (Fritillaria anhuiensis) by acid dye colorimetry. METHODS: The conditions of quantitative analysis were chosen by acid dye colorimetry. RESULTS:The approprite analysis was performed on 2ml 0. 001 Mol L-1bromothymol blue solution,detected at 410 nm. The linearity was established for total alkaloids in the range of 2.0-10.0 ug ?ml-1. CONCLUSION :This method was sensitive,accurate and reproducible. |
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| Keywords: | Fritillaria anhuiensis total alkaloids acid dye colorimetry |
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