| 利用重组人VEGF165制备单克隆抗体的研究 |
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| 引用本文： | 孙大军,杨淑华,李亚萍,杜建时,祁功才.利用重组人VEGF165制备单克隆抗体的研究[J].吉林大学学报(医学版),2001,27(5):475-477. |
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| 作者姓名： | 孙大军 杨淑华 李亚萍 杜建时 祁功才 |
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| 作者单位： | 吉林大学中日联谊医院血管外科 |
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| 摘 要： | 目的 :制备抗重组人血管内皮生长因子 (VEGF) 16 5单克隆抗体。方法 :构建人 VEGF16 5原核表达载体 p ET- 3a/ VEGF16 5 ,并使其在大肠杆菌中高效表达 ,纯化表达产物。用初步纯化的rh VEGF16 5免疫小鼠制成杂交瘤细胞 ,小鼠腹腔接种 ,收集腹水纯化后制成抗 rh VEGF16 5单克隆抗体。结果 :VEGF16 5诱导的内皮细胞增殖可被本研究制备的单克隆抗体中和 ,并表现出较好的剂量依赖关系。结论 :本研究利用 DNA重组技术制备人 VEGF16 5 ,并利用它进一步制备了抗重组人VEGF16 5单克隆抗体。
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| 关 键 词： | 载体 重组人VEGF165 单克隆抗体 |
| 文章编号： | 0253-3707(2001)05-0475-03 |
| 修稿时间： | 2000年9月25日 |
Study of preparing the monoclonal antibody of rhVEGF165 |
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| SUN Da-jun,YANG Shu hua,LI Ya ping ,DU Jian shi,QI Gong cai.Study of preparing the monoclonal antibody of rhVEGF165[J].Journal of Jilin University: Med Ed,2001,27(5):475-477. |
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| Authors: | SUN Da-jun YANG Shu hua LI Ya ping DU Jian shi QI Gong cai |
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| Institution: | SUN Da-jun,YANG Shu hua,LI Ya ping *,DU Jian shi,QI Gong cai |
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| Abstract: | Objective:To prepare the monoclonal antibody of VEGF165.Methods:The recombinant plasmid pET 3a/VEGF165 was highly expressed in E coli.The mouse was immunized with primarily purified rhVEGF165.Then the hybridized tumor was prepared,and then incubated into the peritoneal cavity of mouse.The monoclonal antibody of rhVEGF165 was then prepared after the purification of the gathered ascites. Results: Proliferation of endothelial cell induced by VEGF165 was dose dependently neutralized by the monoclonal antibody prepared in our study.Conclusion:The recombinant human VEGF165 was expressed in E coli . And then the monoclonal antibody of rhVEGF165 was prepared with the expressed protein. |
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| Keywords: | vector rhVEGF165 monoclonal antibody |
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