温州地区健康成人外周血网织红细胞血红蛋白及网织红分群值正常值范围调查

目的 调查温州地区健康成人RET-He及网织红分群值正常值范围.方法 选取2008年8月至2010年2月温州地区1 000名健康体检者,并按性别和年龄分组.按性别分组:男506名,女494名.按年龄分组:20～40岁组341名;＞40～60岁组316名;＞60岁组343名.采集所有调查对象2 ml乙二胺四乙酸二钾(EDTA-K2)抗凝静脉血,利用Sysmex XE-2100五分类自动血细胞分析仪对1 000名健康成人外周血RET-He及网织红分群值(IRF、LFR、MFR、HFR)进行检测,确定其正常值范围.仪器测定血标本之前进行校准和验证,所有标本采集后2 h内完成检测.RET-He、RET绝对值、RET百分数为正态分布数据,采用((x)-1.96 s,(x)+1.96 s)确定正常值范围.网织红分群值(IRF、LFR、MFR、HFR)为偏态分布数据,采用百分位数(P2.5,P97.5)来确定正常值范围.结果 男性RET-He均值为(33.91±1.77)pg,正常值范围为(30.44～37.38)pg,女性均值为(33.97±1.85)pg,正常值范围为(30.34～37.60)pg,差异无统计学意义(t=-0.533,P＞0.05).男性RET绝对值均值为(0.051±0.023)×1012/L,正常值范围为(0.006～0.096)×1012/L,女性均值为(0.037±0.026)×1012/L,正常值范围为(0～0.088)×1012/L,差异有统计学意义(t=8.409,P＜0.05).男性RET百分数均值为(1.041±0.459)%,正常值范围为(0.141～1.941)%,女性均值为(0.869±0.603)%,正常值范围为(0～2.051)%,差异有统计学意义(t=5.074,P＜0.05).男性LFR、MFR、HFR、IRF正常值范围分别为0.951(0.866～0.988)、0.046(0.010～0.114)、0.003(0～0.023)、0.050(0.012～0.134),女性分别为0.096(0.880～0.991)、0.039(0.008～0.113)、0.002(0～0.016)、0.040(0.009～0.121),差异均有统计学意义(Z=-5.044、-4.793、-3.559、-5.075,P均＜0.05).20～40岁组、＞40～60岁组、＞60岁组和全年龄段RET-He均值分别为(33.38±1.49)、(34.36±1.46)、(34.12±2.21)、(33.94±1.81)pg,正常值范围分别为(30.46～36.30)、(31.50～37.22)、(29.79～38.45)、(30.39～37.49)Pg,差异有统计学意义(F=28.072,P＜0.05),且20～40岁组与＞40～60岁组、＞60岁组比较,差异均有统计学意义(q=9.970、7.791,P均＜0.05).＞60岁组LFR、MFR、HFR、IRF正常值范围分别是0.961(0.878～0.992)、0.037(0.007～0.105)、0.002(0～0.020)、0.039(0.008～0.117),与20～40岁组的0.953(0.867～0.990)、0.045(0.009～0.116)、0.003(0～0.019)、0.050(0.010～0.133)比较,差异有统计学意义(Z=-3.949、-4.236、-4.373、-4.973,P均＜0.05),与＞40～60岁组的0.951(0.855～0.989)、0.047(0.010～0.133)、0.003(0～0.020)、0.049(0.011～0.149)比较,差异亦有统计学意义(Z=-2.747、-3.275、-3.901、-4.185,P均＜0.05).RET-He测定值在不同性别组间差异无统计学意义,可以制定同一正常值范围;但RET-He在不同年龄组间差异有统计学意义,应制定不同的正常值范围.LFR、MFR、HFR、IRF在不同性别及不同年龄组间差异均有统计学意义,应按性别和年龄分组制定正常值范围.结论 本研究建立了温州地区健康成人外周血RETHe及网织红分群值正常值范围,提高了网织红细胞参数的临床应用价值.

Abstract：

Objective To investigate the normal ranges of RET-He and reticulocyte grouping parameters of healthy adults in Wenzhou. Methods A total of 1 000 samples from healthy adults were collected in Wenzhou from August 2008 to February 2010. There were 506 males and 494 females. The individuals were stratified based on age into three groups:20 to 40-year-old group (n = 341 ), ＞40 to 60-year-old group (n =316), and ＞60-year-old group (n =343). All blood samples were drawn in EDTA-K2 anticoagulated blood. Peripheral RET-He and reticulocyte grouping parameters ( IRF, LFR, MFR, HFR)were determined by Sysmex XE-2100 automated hematology analyzer. The instrument was calibrated and validated before testing All specimens were analyzed within 2 hours. RET-He, RET absolute value and RET percentage were normal distribution, the normal range was established by using ((x)-1.96s,(x)+ 1.96 s)method. IRF, LFR, MFR and HFR data were skewed distribution,the normal range was established by using (P2.5 ,P97.5 )percentile method. Results The mean and normal range of RET-He were (33.91 ± 1.77) pg and (30.44 - 37.38) pg in male, (33.97 ± 1.85) pg and (30.34 - 37.60) pg in female,respectively. There was no significant difference between the two groups ( t =-0.533, P＞0.05 ). The mean and normal range of RET absolute value were (0.051±0.023) × 1012/L and (0.006 -0.096)×1012/L in male,which were (0. 037 ±0. 026) × 1012/L and (0 -0. 088) × 1012/L in female, there was significant difference between the two groups ( t = 8. 409, P ＜ 0. 05 ) . The mean and normal range of RET percentage in male were(1.041 ±0.459)% and (0. 141 -1.941)% , and in female were (0.869±0.603)% and (0-2. 051 )%, which showed significant difference (t =5.074,P ＜0. 05). The normal ranges of LFR,MFR,HFR,IRF in male were 0. 951 (0.866 -0.988) ,0.046(0.010 -0.114) ,0. 003(0 -0.023) ,0. 050(0.012-0.134), and in female were 0.096(0.880 -0.991) ,0.039(0.008 -0.113) ,0.002(0-0.016) ,0.040(0.009-0.121), respectively. There were significant differences between the two groups (Z values were -5.044, -4.793, -3.559, -5.075, respectively,all P＜0.05). The means of RET-He in 20 to 40-year-old group, ＞40 to 60-year-old group, ＞ 60-year-old group and all age group were (33.38±1.49),(34.36 ±1.46), (34.12±2.21) and (33.94 +1.81) pg, respectively. The normal ranges were(30.46-36.30), (31.50 -37.22), (29.79 - 38.45) and (30.39 - 37.49) pg, which showed statistical difference( F =28. 072,P ＜0. 05). In addition, the normal range of RET-He in 20 to 40-year-old group was lower than that of ＞ 40 to 60-year-old group and ＞ 60-year-old group ( q values were 9. 970, 7. 791,respectively, P＜0. 05). The normal ranges of LFR, MFR, HFR, IRF in ＞ 60-year-old group were 0.961(0. 878 -0. 992) ,0. 037(0. 007 -0. 105 ) ,0. 002(0 -0. 020) and 0. 039(0. 008 -0. 117) ,while they were 0.953(0. 867 -0.990), 0.045(0.009 -0. 116) ,0.003(0 -0.019) ,0. 050(0. 010 -0. 133) in 20 to 40 -year-old group , and 0. 951 (0.855 -0.989) ,0.047 (0.010 - 0.133 ) ,0.003 ( 0-0.020), 0.049 (0.011-0.149)in ＞40 to 60-year-old group, which showed statistical difference (Z values were -3. 949, -4.236,-4. 373, -4. 973, - 2. 747, - 3. 275, - 3.901, - 4. 185, respectively, all P ＜ 0. 05). There was nodifference between male and female for RET-He, the same normal ranges could be used, but there was significant difference between different age groups. LFR, MFR, HFR and IRF were statistically different between different age groups and different sex groups. The normal ranges should be established by gender and age. Conclusion The normal ranges of RET-He and reticulocyte grouping parameters about healthy adults in Wenzhou are established, which plays an important role in clinical value of RET parameters.

Normal ranges of peripreral RET-He and reticulocyte grouping parameters in healthy adults in Wenzhou

Objective To investigate the normal ranges of RET-He and reticulocyte grouping parameters of healthy adults in Wenzhou. Methods A total of 1 000 samples from healthy adults were collected in Wenzhou from August 2008 to February 2010. There were 506 males and 494 females. The individuals were stratified based on age into three groups:20 to 40-year-old group (n = 341 ), ＞40 to 60-year-old group (n =316), and ＞60-year-old group (n =343). All blood samples were drawn in EDTA-K2 anticoagulated blood. Peripheral RET-He and reticulocyte grouping parameters ( IRF, LFR, MFR, HFR)were determined by Sysmex XE-2100 automated hematology analyzer. The instrument was calibrated and validated before testing All specimens were analyzed within 2 hours. RET-He, RET absolute value and RET percentage were normal distribution, the normal range was established by using ((x)-1.96s,(x)+ 1.96 s)method. IRF, LFR, MFR and HFR data were skewed distribution,the normal range was established by using (P2.5 ,P97.5 )percentile method. Results The mean and normal range of RET-He were (33.91 ± 1.77) pg and (30.44 - 37.38) pg in male, (33.97 ± 1.85) pg and (30.34 - 37.60) pg in female,respectively. There was no significant difference between the two groups ( t =-0.533, P＞0.05 ). The mean and normal range of RET absolute value were (0.051±0.023) × 1012/L and (0.006 -0.096)×1012/L in male,which were (0. 037 ±0. 026) × 1012/L and (0 -0. 088) × 1012/L in female, there was significant difference between the two groups ( t = 8. 409, P ＜ 0. 05 ) . The mean and normal range of RET percentage in male were(1.041 ±0.459)% and (0. 141 -1.941)% , and in female were (0.869±0.603)% and (0-2. 051 )%, which showed significant difference (t =5.074,P ＜0. 05). The normal ranges of LFR,MFR,HFR,IRF in male were 0. 951 (0.866 -0.988) ,0.046(0.010 -0.114) ,0. 003(0 -0.023) ,0. 050(0.012-0.134), and in female were 0.096(0.880 -0.991) ,0.039(0.008 -0.113) ,0.002(0-0.016) ,0.040(0.009-0.121), respectively. There were significant differences between the two groups (Z values were -5.044, -4.793, -3.559, -5.075, respectively,all P＜0.05). The means of RET-He in 20 to 40-year-old group, ＞40 to 60-year-old group, ＞ 60-year-old group and all age group were (33.38±1.49),(34.36 ±1.46), (34.12±2.21) and (33.94 +1.81) pg, respectively. The normal ranges were(30.46-36.30), (31.50 -37.22), (29.79 - 38.45) and (30.39 - 37.49) pg, which showed statistical difference( F =28. 072,P ＜0. 05). In addition, the normal range of RET-He in 20 to 40-year-old group was lower than that of ＞ 40 to 60-year-old group and ＞ 60-year-old group ( q values were 9. 970, 7. 791,respectively, P＜0. 05). The normal ranges of LFR, MFR, HFR, IRF in ＞ 60-year-old group were 0.961(0. 878 -0. 992) ,0. 037(0. 007 -0. 105 ) ,0. 002(0 -0. 020) and 0. 039(0. 008 -0. 117) ,while they were 0.953(0. 867 -0.990), 0.045(0.009 -0. 116) ,0.003(0 -0.019) ,0. 050(0. 010 -0. 133) in 20 to 40 -year-old group , and 0. 951 (0.855 -0.989) ,0.047 (0.010 - 0.133 ) ,0.003 ( 0-0.020), 0.049 (0.011-0.149)in ＞40 to 60-year-old group, which showed statistical difference (Z values were -3. 949, -4.236,-4. 373, -4. 973, - 2. 747, - 3. 275, - 3.901, - 4. 185, respectively, all P ＜ 0. 05). There was nodifference between male and female for RET-He, the same normal ranges could be used, but there was significant difference between different age groups. LFR, MFR, HFR and IRF were statistically different between different age groups and different sex groups. The normal ranges should be established by gender and age. Conclusion The normal ranges of RET-He and reticulocyte grouping parameters about healthy adults in Wenzhou are established, which plays an important role in clinical value of RET parameters.