Establishment of gene-vaccinated skin grafting against Toxoplasma gondii infection in mice |
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Authors: | Saito S Aosai F Rikihisa N Mun H S Norose K Chen M Kuroki T Asano T Ochiai T Hata H Ichinose M Yano A |
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Institution: | Department of Plastic and Reconstructive Surgery, Chiba University Hospital, Chiba University School of Medicine, 260-8670, Chiba, Japan. |
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Abstract: | Vaccine effects of in vivo gene-vaccinated skin graft were evaluated against Toxoplasma gondii (T. gondii) infection. By using a gene gun, cDNA coding T. gondii SAG1 molecule was intracutaneously vaccinated into C57BL/6 (B6; a susceptible strain), BALB/c (a resistant strain) and (C57BL/6 x BALB/c) F1 (CBF1) mice, and the gene-vaccinated skin of these strains was transplanted to CBF1 mice. Regarding the antibody production against SAG1, CBF1-recipient mice transplanted with the SAG1 gene-vaccinated B6 skin were high responders, whereas CBF1 mice skin grafted with vaccinated skin of both BALB/c and CBF1 mice were low responders. The donor-derived LC/DC migrated to the draining lymph nodes of the recipients from the skin graft within 3 days. The vaccine effect against T. gondii challenge infection was obtained in CBF1 mice which received the skin graft of the SAG1 gene-vaccinated BALB/c mice. |
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