TB—Ab和ADA联合检测在结核性胸腔积液104例中的诊断价值

目的：探讨结核抗体（TB—Ab）和腺苷脱氨酶（ADA）在胸腔积液中的鉴别诊断价值。方法：应用斑点金免疫渗滤试验（DIG—FA）分别检测胸腔积液和血清中的TB～Ab；应用腺苷脱氨酶偶联谷氨酸脱氢酶连续检测法测定胸水及血清中的ADA活性，并运用统计学方法对数据进行处理。结果：结核性胸腔积液患者胸水和血清中TB—Ab的阳性率分别为65．3％和72．1％，与恶性胸腔积液患者TB—Ab的阳性率（11．3％和13．2％）相比有显著性差异（P〈0．01）；结核性胸腔积液ADA活性明显增高（64．37±22．41）与恶性胸腔积液ADA活性（16．23±6．26）相比有显著性差异（P〈0．01）；两者血清ADA活性无显著性差异（P〉0．05）。结核组ADA胸水／血清〉1（4．23±2．98），非结核组胸水／血清〈1（0．69±0．22），两组相比有显著性差异（P〈0．01）。结论：TB—Ab和ADA联合检测在结核性胸腔积液的诊断中具有重要的价值。

Differentiation diagnostic value of TB -Ab and ADA measurement in Tuberculous Exudates

Objective： To evaluate the differential diagnosis value of measurement of Mycobacterium tuberculosis antibody （ TB - Ab） and adenosine deaminase （ADA） in tuberculous exudates. Methods ： The TB - Ab in pleural effusion and serum were measured by DIGFA, ADA were examined by method of continuous monitoring with enzyme. Results： The positive rates of TB -Ab in pleural effusion and in serum of 104 cases with tuberculosis were 65.3% and 72.1% respectively, which of 53 malignant exudates were 11.3% and 13.2% respectively. The ADA activity of tuberculosis and malignant exudates were respectively （64.37 ± 22.41 ） U/L and （16.23 ± 6.26）U/L . The p- ADA/s- ADA of tuberculosis and malignant exudates were respectively （4.23 ± 2.98 ） and （0.69 ± 0.22 ）. Conclusions ： The measurement of TB - Ab and ADA in pleural exudates and serum is of great value in differentiating tuberculous exudates.