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| 丹参DNA提取方法的优化 | |
| 引用本文： | 王春丽,梁宗锁,李殿,王灏,舒志明.丹参DNA提取方法的优化[J].药物生物技术,2007,14(1):29-33. |
| 作者姓名： | 王春丽 梁宗锁 李殿 王灏 舒志明 |
| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西,杨凌,712100;陕西省杂交油菜研究中心,陕西,大荔,715105 2. 西北农林科技大学,生命科学学院,陕西,杨凌,712100 3. 陕西省杂交油菜研究中心,陕西,大荔,715105 |
| 基金项目： | 教育部跨世纪优秀人才培养计划;西北农林科技大学拔尖人才支持项目 |
| 摘 要： | 针对药用植物丹参含有较多的多酚类、醌类等水溶性次生代谢物，在提取DNA时易发生共沉淀而很难除去等问题，设计双蒸水预洗不同次数和活性碳吸附杂质等处理，对CTAB和SDS两种DNA提取方法进行了系统优化。研究得出，采用2％的CTAB提取液和双蒸水预洗一遍并且添加样品鲜重4％的活性碳于提取液中的提取方法最佳。不仅能除去多酚类、醌类等次生代谢物，而且去多糖、蛋白质效果良好，所获DNA纯度高；RAPD-PCR实验结果稳定清晰可靠。 |
| 关 键 词： | 丹参 次生代谢物 双蒸水预洗 活性炭 |
| 文章编号： | 1005-8915（2007）01-0029-05 |
| 修稿时间： | 2006-08-182006-10-12 |
Optimization of DNA Extraction Methods from Salvia Miltiorrhiza Bge |
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| WANG Chun-li,LIANG Zong-suo,LI Dian-rong,WANG Hao,SHU Zhi-ming.Optimization of DNA Extraction Methods from Salvia Miltiorrhiza Bge[J].Pharmaceutical Biotechnology,2007,14(1):29-33. | |
| Authors: | WANG Chun-li LIANG Zong-suo LI Dian-rong WANG Hao SHU Zhi-ming |
| Abstract: | Salvia Miltiorrhiza Bge contains a plenty of secondary metabolites, such as polyphenol deri-vatives and quinonoid compounds which could combine DNA to form sediments and are hard to be removed. On the basis of optimization for pre-wash times with redistilled water and proportions of charcoal added, two DNA-extraction methods of CTAB and SDS were compared. The results showed that the method (2% CTAB solution, pre-wash once and 4% of weight of fresh samples for charcoal) was optimal. This method has the advantages that not only polyphenol and quinonoid compounds, but also polysaccharide and proteins are eliminated. As a result, high purity DNA was obtained to be used as the model of RAPD-PCR and the electrophoretogram of DNA amplification product was clear and credible in this research. |
| Keywords: | CTAB |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |