反相离子对高效液相色谱法测定柿叶和柿蒂中的熊果酸与齐墩果酸

目的:建立反相离子对高效液相色谱法( Ion-pair RP-HPLC)测定柿叶和柿蒂中的熊果酸与齐墩果酸,并考察其药用价值.方法:采用AT Lichrom C18色谱柱(150 mm×4.6 mm,5μm)；以甲醇-0.78％四丁基氢氧化铵水溶液(85:15)为流动相；检测波长为210 nm;流量为0.8 ml ·min-1；柱温为室温；进样量为20μl.结果:熊果酸与齐墩果酸在进样量为0.25 ～2.0μg时,线性关系良好(R2分别为0.9992,0.9994).熊果酸平均回收率为98.80％,RSD为1.34％；齐墩果酸平均回收率为100.36％,RSD为2.04％.结论:该法操作简便、准确、重现性好.

Determination of Ursolic Acid and Oleanolic Acid in Persimmon Leaves and Stems by Ion pair RP-HPLC

Objective：To establish a method for the determination of ursolic acid and oleanolic acid in persimmon leaves and stems by ion-pair RP-HPLC to examine its medicinal value.Method：Chromatographic conditions included an AT Lichrom C_（18）（150 mm×4.6 mm,5μm） column and the mobile phase of methanol - 0.78%tetrabutyl ammonium hydroxide water solution（85：15 ）. The detection wavelength was at 210 nm,the flow rate was 0.8 ml -min~（-1）,the column temperature was ambient and the injection volume was 20μl.Result：Within the range of 0.25～2.0μg,the calibration curves were linear for ursolic acid and oleanolic acid（R~2 = 0.999 2,0.999 4）.The average recovery of ursolic acid was 98.80%with RSD of 1.34%,and that of oleanolic acid was 100.36% with RSD of 2.04%.Conclusion：This method is simple and accurate with good reproducibility.