铝离子对K562细胞红系分化能力抑制作用

目的 研究铝离子对K562细胞红系分化能力的影响.方法 应用台盼蓝染色排除法分析铝离子对K562细胞的活力的影响;应用联苯胺染色法检测铝离子作用下K562细胞的红系分化率;利用荧光标记抗体结合流式细胞技术分析铝离子处理K562细胞的细胞表面转铁蛋白受体CD71的表达.结果 50、100和150μmol/L铝离子作用24～72 h对K562细胞生长有浓度依赖性抑制作用,各浓度组作用72 h时的相对活力分别为(89.23±4.37)%、(52.78±1.58)%和(44.69±1.95)%;铝离子作用48 h对氯化高铁血红素诱导的K562细胞血红蛋白合成表现出较为明显的浓度依赖性抑制,相对于对照组细胞,各浓度组的血红蛋白合成抑制率分别为3.37%、8.61%和10.55%;经100μmol/L铝离子处理48 h后的K562细胞表面CD71的表达水平增加27.7%.结论 一定浓度的铝离子对K562细胞的红系分化能力有抑制作用.

Effect of aluminum on erythroid differentiation of K562 cells

Objective To explore the effect of aluminum chloride on the erythroid differentiation of K562 cells.Methods The percentage of viable cells was determined by trypan blue staining.The percentage of cells containing hemoglobin was estimated by staining with benzidine.The expression of transferrin receptor CD71 on the surface of K562 cells was estimated by flowcytometry after staining with FITC-conjugated anti-CD71 antibody.Results After the K562 cells were treated with Al(50-150 μmol/L) for 24,48,or 72 hours,the growth of K562 cells showed a concentration-dependent inhibition.When K562 cells were exposed to Al(50-150 μmol/L) for 48 hours,the hemin-induced hemoglobin synthesis showed an obvious concentration-dependent inhibition.The exposure to aluminum ions(100 μmol/L) for 48 hours caused an up-regulation of CD71 protein on the cell surface.Conclusion Aluminum chloride could inhibit hemin-induced erythroid differentiation of the K562 cells,suggesting that K562 cells could be used as a in vitrol model to research the mechanisms of the erythropoietic toxicity of aluminum.