| 叶酸对柯萨奇病毒B3诱导乳鼠心肌凋亡保护作用 |
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| 引用本文： | 王晓琼,仇小强,张明,钟秋安,谭盛葵,邓莹莹.叶酸对柯萨奇病毒B3诱导乳鼠心肌凋亡保护作用[J].中国公共卫生,2011,27(9). |
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| 作者姓名： | 王晓琼 仇小强 张明 钟秋安 谭盛葵 邓莹莹 |
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| 作者单位： | 桂林医学院附属医院心内科,广西 桂林,541001 |
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| 基金项目： | 广西自然科学基金重点项目，广西自然科学基金 |
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| 摘 要： | 目的 探讨叶酸对柯萨奇病毒B3诱导的乳鼠心肌细胞凋亡及凋亡相关基因与蛋白表达的影响.方法 取成熟健康雌性Wistar大鼠随机分为4组,分别为对照、叶酸、柯萨奇病毒B3及柯萨奇病毒B3+叶酸组；孕前灌胃给予叶酸2周,孕第5d腹腔注射给予柯萨奇病毒B3,连续5d；取自然分娩乳鼠心肌细胞,切片观察乳鼠心肌损伤；原位末端标记法( tunel)检测乳鼠心肌细胞凋亡；蛋白免疫印迹(western blot)及逆转录聚合酶链反应(RT-PCR)测定乳鼠心肌细胞凋亡相关Bax、Bcl-2、Caspase-3蛋白及基因表达.结果 柯萨奇病毒组乳鼠心肌细胞损伤明显,凋亡细胞增加,加用叶酸后状态改善；病毒组乳鼠心肌细胞Bax、Caspase-3蛋白表达(0.608±0.060,0.951±0.038)及基因表达(0.951 ±0.098,0.960±0.074)高于病毒+叶酸组,病毒组Bcl-2蛋白表达(0.350±0.037)及基因表达(0.423±0.051)低于病毒+叶酸组.结论 怀孕母鼠早期感染柯萨奇病毒可致乳鼠心肌细胞凋亡增加,补充叶酸对乳鼠心肌细胞凋亡具有明显保护作用.
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| 关 键 词： | 叶酸 柯萨奇病毒B3 心脏细胞 细胞凋亡 |
Prtoective effact of folic acid on myocardial apoptosis of offspring rats with maternal coxsackievirus B3 infection |
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| WANG Xiao-qiong,QIU Xiao-qiang,ZHANG Ming,et al..Prtoective effact of folic acid on myocardial apoptosis of offspring rats with maternal coxsackievirus B3 infection[J].Chinese Journal of Public Health,2011,27(9). |
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| Authors: | WANG Xiao-qiong QIU Xiao-qiang ZHANG Ming |
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| Institution: | WANG Xiao-qiong,QIU Xiao-qiang,ZHANG Ming,et al.Department of Cardiovascular Internal Medicine,Guilin Medical University(Guilin 541004,China) |
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| Abstract: | Objective To investigate the effects of oral folic acid on myocardial apoptosis of offspring in an experimental model of coxsackievirus B3(CVB3) infection of pregnant rats.Methods Wistar rats were randomly divided into four groups.Female rats in each group were copulated with normal male rats.After natural birth,the hearts of neonates were taken and stored in liquid nitrogen.Myocardial pathological changes were observed for the neonatal rats.Myocardial apoptosis was quantitated with terminal doxynucleotidyl... |
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| Keywords: | folic acid coxsackievirus B3 cell apoptosis |
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