| HPLC法测定复方苯妥英钠凝胶剂中苯妥英钠的含量 |
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| 引用本文： | 刘超英,张慧慧. HPLC法测定复方苯妥英钠凝胶剂中苯妥英钠的含量[J]. 中国药师, 2012, 15(3): 345-347 |
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| 作者姓名： | 刘超英 张慧慧 |
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| 作者单位： | 湖北中医药大学药学院 武汉 430061 |
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| 基金项目： | 湖北省教育厅资助项目（D20112006） |
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| 摘 要： | 目的:建立高效液相色谱法测定复方苯妥英钠凝胶剂中苯妥英钠的含量.方法:采用ZORBAX SB-C18色谱柱(250 mm×46 mm,5μm)；流动相为0.05 mol·L-1磷酸二氢铵(用磷酸调pH 2.5)-乙腈-甲醇(45:35:20)；流速0.8 ml ·min-1；检测波长220 nm;柱温:25℃结果:苯妥英钠在18.012-42.028μg·ml-1浓度范围内线性关系良好(r =0.999 0)；平均回收率为100.2％(RSD =0.45％,n=9).结论:本方法简单,灵敏,重复性好,可用于复方苯妥英钠凝胶剂中苯妥英钠的含量测定.
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| 关 键 词： | 苯妥英钠 凝胶剂 高效液相色谱法 |
| 收稿时间： | 2011-09-25 |
| 修稿时间： | 2011-11-19 |
Determination of Phenytoin Sodium in Compound Phenytoin Sodium Gels by HPLC |
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| Liu Chaoying and Zhang Huihui. Determination of Phenytoin Sodium in Compound Phenytoin Sodium Gels by HPLC[J]. China Pharmacist, 2012, 15(3): 345-347 |
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| Authors: | Liu Chaoying and Zhang Huihui |
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| Affiliation: | College of Pharmacy, Hubei University of Chinese Medicine ,Wuhan 430061;College of Pharmacy, Hubei University of Chinese Medicine ,Wuhan 430061 |
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| Abstract: | Objective：To establish an HPLC method for the determination of phenytoin sodium in compound phenytoin sodium gels.Method：The HPLC analysis was carried out on a ZORBAX SB-C_（18）（250 mm×46 mm,5μm）column with NH_4H_2PO_4（0.05 mol·L~- ） -acetonitrile- methanol（45：35：20）（adjusting pH to2.5 with phosphoric acid） as the mobile phase at the flow rate of 0.8 ml·min~（-1）.The detection wavelength was 220 nm and the column temperature was 25℃.Result：The linear range of phenytoin sodium was 18.012-42.028μg·ml~（-1）.The average recovery was 100.2%（RSD =0.45%,n =9）.Conclusion：The method is simple,sensitive and reproducible,and can be used in the determination of phenytoin sodium in compound phenytoin sodium gels. |
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| Keywords: | Phenyoin sodium Gels HPLC |
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