| 孕期染毒双酚A对雄性子代生殖发育影响 |
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| 引用本文： | 段志文,闻霞,王超,张玉敏,裴秀丛,马明月. 孕期染毒双酚A对雄性子代生殖发育影响[J]. 中国公共卫生, 2011, 27(9) |
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| 作者姓名： | 段志文 闻霞 王超 张玉敏 裴秀丛 马明月 |
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| 作者单位： | 1. 沈阳医学院公共卫生学院毒理学教研室,辽宁沈阳,110034
2. 沈阳市第五人民医院 |
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| 基金项目： | 辽宁省教育厅重点实验室项目 |
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| 摘 要： | 目的 探讨母鼠孕期接触双酚A对子代雄鼠生殖发育的影响及作用机制.方法 将受孕SD雌鼠40只,随机分为双酚A0、10、50、250 mg/kg染毒组,以2 mL/kg容积自受孕第5d灌胃染毒至第20d；孕第21 d每组处理5只孕鼠,检测母鼠血中性激素水平,胎鼠数、死胎数、胎鼠重和胎盘重,高效液相色谱法检测母鼠血及胎盘中双酚A含量,另5只孕鼠自然分娩,待雄性子鼠性成熟后检测血中性激素水平,生殖器官脏器系数、病理、精子质量.结果 10、50 mg/kg剂量组母鼠血清中雌二醇(E2)含量分别为1 780.78、1 680.10 pmol/L,明显高于对照组的1361.74pmol/L(P＜0.01),低、中、高3个染毒组睾酮(T)含量分别为5.55、4.76、6.79 nmol/L,明显高于对照组的3.20 nmol/L(P＜0.01)；50、250mg/kg剂量组雄性子鼠附睾及前列腺脏器系数分别为0.297、0.325和0.153、0.173,均高于对照组(P＜0.01),镜下可见附睾间质及前列腺上皮有增生改变；250 mg/kg剂量组精子活动率为52.9%,明显低于对照组的67.4% (P ＜0.05).结论 孕期染毒双酚A对胎鼠发育无明显影响,但影响子鼠生殖器官发育和精子生成质量.
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| 关 键 词： | 孕期 双酚A 生殖发育 |
Effect of maternal exposure to bisphenol A during pregnancy on reproductive development in F1 male rats |
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| DUAN Zhi-wen,WEN Xia,WANG Chao,et al.. Effect of maternal exposure to bisphenol A during pregnancy on reproductive development in F1 male rats[J]. Chinese Journal of Public Health, 2011, 27(9) |
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| Authors: | DUAN Zhi-wen WEN Xia WANG Chao et al. |
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| Affiliation: | DUAN Zhi-wen,WEN Xia,WANG Chao,et al.Department of Toxicology,School of Public Health,Shenyang Medical College(Shenyang 110034,China) |
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| Abstract: | Objective To observe the growth and development of F1 male fetus and changes in reproductive organs of the rats after maternal exposure to bisphenol A(BPA) and to explore the effect of maternal exposure to BPA during pregnancy on the reproductive development in F1 male rats.Methods Forty pregnant Sprague-Dawley rats were randomed into 4 groups,including vehicle control(corn oil)and BPA treatment groups(10,50,and 250 mg/kg,respectively) with the volume of 2 ml/kg.The rats were gavaged once a day from the 5th... |
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| Keywords: | pregnant period bisphenol A reproductive development |
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