| HPLC法测定养咽合剂中黄芩苷的含量 |
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| 引用本文： | 胡大强,杨晓军,傅若秋,孟德胜,卢来春,徐靖,王林丽. HPLC法测定养咽合剂中黄芩苷的含量[J]. 中国药师, 2012, 15(3): 332-334 |
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| 作者姓名： | 胡大强 杨晓军 傅若秋 孟德胜 卢来春 徐靖 王林丽 |
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| 作者单位： | 1. 第三军医大学第三附属医院药剂科 重庆400042
2. 蚌埠医学院药学系 |
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| 基金项目： | 重庆市卫生局中医药科研计划项目（编号：2010-3-3） |
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| 摘 要： | 目的:建立高效液相色谱法测定养咽合剂中黄芩苷含量的方法.方法:采用高效液相色谱法,色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸-三乙胺(60∶40∶0.2∶0.2),进样量为10μl,检测波长为274 nm,流速为1.0ml·min-1,柱温30℃.结果:黄芩苷在2.4～15.0 μg ·ml-1范围内与峰面积呈良好的线性关系(r =0.999 4),平均加样回收率为101.60％,RSD=0.84％(n=9).结论:本方法简便、准确、分离效果好,可用于养咽合剂中黄芩苷的含量测定.
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| 关 键 词： | 黄芩苷 养咽合剂 高效液相色谱法 |
| 收稿时间： | 2011-09-29 |
| 修稿时间： | 2011-12-12 |
Content Determination of Baicalin in Yangyan Mixtures by HPLC |
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| Hu Daqiang,Yang Xiaojun,Fu Ruoqiu,Meng Desheng,Lu Laichun,Xu Jing and Wang Linli. Content Determination of Baicalin in Yangyan Mixtures by HPLC[J]. China Pharmacist, 2012, 15(3): 332-334 |
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| Authors: | Hu Daqiang Yang Xiaojun Fu Ruoqiu Meng Desheng Lu Laichun Xu Jing Wang Linli |
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| Affiliation: | 1.Department of pharmacy,the Third Affiliated Hospital of the Third Military Medical University,Chongqing 400042,China;2.Department of Pharmacy,Bengbu Medical college ) |
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| Abstract: | Objective：To establish a method for the content determination of baicalin in Yangyan mixtures.Method：An HPLC method was applied.The sample was separated on a Diamonsil C_（18）（250 mm×4.6 mm,5μm）column with methanol-water-phosphoric acid-triethylamine（60：40：0.2：0.2） as the mobile phase at a flow rate of 1.0 ml·min~（-1）.The detection wavelength was set at 274 nm and the column temperature was 30℃.Result：The linear range of baicalin was 2.4-15.0μg·ml~（-1）（r=0.999 4）with average recovery of 101.60（RSD =0.84%,n =9）.Conclusion：The method is simple and accurate with promising separation for the content determination of Yangyan mixtures. |
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| Keywords: | Baicalin Yangyan mixtures HPLC |
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