教学用话筒头部球面微生物检测结果分析

目的 检测教学用话筒头部球面微生物分布情况.方法无菌棉拭子采集标本,采用常规细菌培养与鉴定法检测细菌,采用直接免疫荧光法(DFA)检测多种呼吸道病毒抗原,采用聚合酶链反应(PCR)法对肺炎支原体、肺炎衣原体、沙眼农原体的特异性DNA核酸片段进行检测.结果话筒头部球面细菌学检出2～6个菌属,3～9个菌种,以葡萄球菌属、链球菌属、奈瑟菌属、棒状杆菌属、芽胞杆菌属为主,分别占17.33％ ～ 59.77％、21.21％ ～88.89％、15.78％～23.54％、5.67％ ～ 11.12％、2.08％～12.36％；其中2株鉴定为金黄色葡萄球菌；病毒学抗原检出腺病毒、呼吸道合胞病毒、流感病毒A型、流感病毒B型、副流感病毒1型、副流感病毒2型、副流感病毒3型检出率分别为56.25％、87.50％、68.75％、81.25％、43.75％、43.75％、68.75％,PCR检出沙眼衣原体阳性1例,阳性率为6.25％；肺炎支原体、肺炎农原体未检出阳性结果.结论多媒体教学用话筒头部球体表面存在致病性微生物与条件致病性微生物,是一种潜在的呼吸道感染传播媒介.

Distribution of microorganism on surface of multimedia microphone head

Objective To investigate the variation and distribution of microorganism on the surface of microphone head.Methods The specimens were collected with sterilized swab scrubbing.The abundance and density of the microorganisms were detected with bacterial culture and identification tests.The antigen of respiratory virus was detected with direct fluorescence antibody(DFA) and the specific DNA of Mycoplasma pneumoniae,Chlamydiae trachomatis,and Chlamydophila pneumoniae were detected with polymerase chain reaction...