凋亡因子在缺氧缺水缺食致心脏损伤中作用

目的 探讨凋亡相关因子Bax、Bcl-2、细胞色素C和caspase-3在缺氧缺水缺食条件下大鼠心肌细胞损伤中变化及其意义.方法将80只Wistar大鼠置于常压低氧舱(10％氧浓度)并禁食水,于实验1、3、5、7d,采用原位末端标记、免疫组织化学和原位杂交技术等方法,检测缺氧缺水缺食后心脏凋亡指数变化及凋亡相关因子Bax、Bcl-2、细胞色素C和caspase-3在其中的作用.结果 10％缺氧复合缺水缺食后1d和7d大鼠心肌细胞凋亡指数(62.63±14.97)、(20.19±3.26)增加；Bax、细胞色素C和caspase-3蛋白在单纯缺氧和缺氧缺水缺食后均可见1～7d表达增加,缺水缺食后在3～7d表达增加.Bcl-2在单纯缺氧和缺水缺食后1～7 d表达减少,缺氧缺水缺食后1～3d表达减少；Caspase-3 mRNA在单纯缺氧组和缺氧缺水缺食后1 d(4.19±1.42)、(3.87±0.86)和7 d(10.43±2.79)、(12.12 +2.27)表达增加,缺水缺食后1 d(3.85±0.88)表达增加.结论10％缺氧复合缺水缺食可引起心肌细胞凋亡,与Bax表达增加、Bcl-2表达减少有关；可能进一步通过线粒体途径,释放细胞色素C,激活caspase-3,引发心肌细胞凋亡.

Role of apoptosis factors in heart injury induced by hypoxia combined with water and dietary depletion in rats

Objective To investigate the change of apoptosis factors Bax,Bcl-2,cytochrome C,and caspase-3 and their role in the mechanism of myocardial cells injury under hypoxia combined with water and dietary depletion in rats.Methods Eighty wistar rats were placed in a self-designed normobaric hypoxic chamber with the oxygen concentration of 10%.In situ cell apoptosis detection,immunochemistry and in situ hybridization were used to detect the change of apoptosis index of myocardial cells and the expressions of Bax,B...