女贞子超临界CO2萃取物遗传和亚急性毒性评价

目的 从遗传毒性和亚急性毒性角度对女贞子超临界CO²萃取物进行毒理学评价.方法 采用测定小鼠骨髓微核率和大鼠30 d饲养试验对受试动物进行体重、血液学指标、血清生化指标和脏器病理检测.结果 在骨髓微核试验中,添加女贞子超临界CO²萃取物各剂量组的骨髓微核率与阴性对照组比较,差异均无统计学意义(P>0.05),但与阳性对照组比较,差异均有统计学意义(P<0.01);在30 d饲养试验中,添加女贞子超临界CO²萃取物的各剂量组动物在给药期均存活,且采食量、体重、行为活动性状等均正常;血液学检测结果,白细胞数9.3×10¹²～10¹²/L、红细胞数8.23×10⁹～8.89×10⁹/L、血红蛋白136.4～140.9 g/L和血小板312.3～330.9×10⁹/L,血液生化指标检测结果,谷丙转氨酶41.8～45.8μ/L,谷草转氨酶150.3～153.0μ/L,尿素氮4.8～5.1 mmol/L,肌酐104.5～109.5μmol/L,血糖5.0～5.49 mmol/L,血清白蛋白38.87～40.8 g/L,总蛋白84.3～89.1 g/L,总胆固醇1.4～1.5 mmol/L和甘油三酯0.81～0.87 mmol/L,主要脏器重量和脏器系数均在正常范围内.结论 女贞子超临界CO²萃取物不能引起小鼠遗传突变和大鼠亚慢性中毒.

Genetic toxicity and subacute toxicity tests of supercritical CO2 extract of Ligustrum lucidum in rats

Objective To study the safety of supercritical CO2 extract of Ligustrum lucidum with a series of gengtic toxicity and subacute toxicity tests. Methods The genetic toxicity was tested with mouse micronucleus test and subacute toxicity test by 30-day feeding test in rats. Results There was no significant difference in the micronucleus test between low,moderate and high dose treated mice and the negative control group(P>0.05).But compared to that of the positive group,micronucleus rate was significantly differ...