广东高本底辐射地区老年居民淋巴细胞永生化细胞系的建立

目的 建立辐射高本底地区老年居民外周血永生化细胞系,以保存高本底居民特有的基因组资源,为进一步开展天然本底辐射的遗传学和分子生物学研究提供材料。方法 在知情同意的情况下收集20例高本底地区老年男性居民的外周血样品,采用EB病毒转化技术,把外周血B淋巴细胞转化成永生化淋巴母细胞系,冻存复苏后,进行细胞活性检测和排除细菌、支原体污染,并用遗传学方法检测其建系前后的遗传稳定性。结果 成功建立20例40株细胞株,所有建成的细胞株冻存后复苏成功率为100%。活性细胞大于90%,无细菌和支原体污染,G显带分析结果为转化后第20代细胞的染色体核型未发生改变。结论 本研究建立的高本底地区老年居民永生细胞系在遗传学特性上是稳定的,可用于进一步的天然本底辐射的遗传学和分子生物学研究。

Establishment of immortalized B lymphoblastoid cell lines of old residents in high background radiation area in Guangdong, China

Objective To establish the immortalized cell lines of peripheral blood lymphocytes for old male residents in high background radiation area (HBRA) in Guangdong, China, in order to preserve the specific genomic resources of residents in HBRA for the further genetic and molecular biological study on HBRA.Methods The peripheral blood samples of 20 old male residents in HBRA were collected after informed consent. The immortalized B lymphoblastoid cell lines, 2 for each resident, were established with Epstein-Barr virus. After being frozen and recovered, the cell viability, the contamination of bacterium and mycoplasma were analyzed. The stabilization of cell lines was decided by comparing the karyotypes of the peripheral blood lymphocytes and the cell lines.Results 40 cell lines for 20 residents in HBRA were successfully established. The recovery rate of cell lines after being frozen was 100%. All the cell viablity after recovery was higher than 90%, and no contamination of bacteria and mycoplasma occurred. The karyotypes of the 20th generation cell lines were not change.Conclusion The immortalized cell lines established in this study could provide biological resources for further study on genetics and molecular biology in HBRA.