肠道SIgA含量的变化与溃疡性结肠炎相关性的分析研究

目的：在建立Wistar大鼠溃疡性结肠炎（UC）模型基础上，探讨肠道SIgA含量的变化与溃疡性结肠炎发病的相关性。方法：采用饮用DSS（Dextran sulfacte sodium）水溶液，建立UC动物模型，随机分为3组，即奥沙拉嗪治疗组（A组）：2次／d奥沙拉嗪（Olsalazing）150mg／kg灌胃；模型组（B组）：UC模型大鼠每天饮用生理盐水；另设正常对照组（C组）：每天饮用生理盐水。采用放射免疫分析法检测肠内容物分泌型IgA（SIgA）含量。结果：DSS所致大鼠UC模型简单易行与人类病变相类似。模型组SIgA含量较正常对照组明显降低（P〈0．05），经奥沙拉嗪治疗后有明显回升，与模型组比较有显著差异（P〈0．05），治疗组与正常对照组比较无差异（P〉0．05）。结论：SIgA含量的变化与溃疡性结肠炎的发生有相关性的关系。

Study of correlation between variation of intestinal SIgA content and ulcerative colitis

Objective:To establish a rat model of ulcerative colitis(UC) and to study the correlation between variation of intestinal SIgA content and pathogenesis of UC.Methods:Rat model of ulcerative colitis was established by oral administration of dextrane sulfate sodium(DSS) solution.These model rats were divided randomly into three groups.Treatment group(group A) was given olsalazing 150 mg/kg via intragastric administration twice a day,animal model group(group B) was given normal sodium(NS) and normal control group were given NS.Radioimmune assay was used to measure the level of SIgA in colonal contents.Results:Rat model of UC was successfully established and it resembled to human UC.The level of SIgA of group B was lower than that of the normal control group(P<0.05).After treated with olsalazing,the content of SIgA increased significantly(P<0.05) and was similar to that of the normal control group.Conclusion:The variation of intestinal SIgA content is correlated with the genesis of UC.