丹参多酚酸盐负性调节小鼠骨髓来源树突状细胞成熟及其部分免疫功能

目的:观察丹参多酚酸盐对小鼠骨髓来源树突状细胞(bone marrow-derived dendritic cells,BMDC)成熟及其部分免疫学功能的影响。方法:小鼠BMDC体外培养时加入促成熟剂脂多糖(DCLPS)或同时加入脂多糖及丹参多酚酸盐(DCSV),其中丹参多酚酸盐设3个浓度:低浓度50μg/ml(SVL)、中浓度100μg/ml(SVM)、高浓度200μg/ml(SVH)。流式细胞仪分析各组细胞表面MHC-Ⅱ分子及共刺激分子CD40、CD80、CD86的表达;FITC-Dextran内吞实验分析各组DC抗原吞噬功能;酶联免疫吸附实验(enzyme linked i mmunosorbent assay,ELISA)检测各组DC培养液上清白细胞介素(interleukin,IL)-12 p40水平。3H]-TdR掺入法检测各组DC体外混合淋巴细胞反应(mixed lymphocyte reactions,MLR)刺激同种反应性T细胞的增殖能力;ELISA法测定MLR上清IL-10、IL-2、干扰素(interferon,IFN)-γ水平。结果:与DCLPS相比,SVL、SVM、SVH细胞表面M...

Salvianolate negatively regulates maturation and immunologic functions of mouse bone marrow-derived dendritic cells

Objective: To observe the effect of salvianolate on the maturation and immunologic functions of mouse bone marrow-derived dendritic cells (BMDCs). Methods: Mouse BMDCs were cultured with GM-CSF and IL-4 for 5 days to obtain immature DCs (imDCs),then the imDCs were cultured in the presence of LPS (1 μg/ml) or LPS (1 μg/ml) plus salvianolate (50,100 and 200 μg/ml) for 48 h to obtain DC_(LPS), SV_L , SV_M and SV_H. The immunophenotypes and antigen phagocytosis capabilities of imDCs, DC_(LPS) , SV_L, SV_M and SV_H were analyzed by flow cytometry and FITC-Dextran intake. The IL-12 p40 contents in the supernatants were determined by enzyme linked immunosorbent assay (ELISA). The stimulatory activities of imDCs,DC_(LPS) ,SV_L,SV_m and SV_H on allogencic T cells in mixed lymphocyte reactions (MLR) were tested by incorporation of 3~H-TdRj the contents of IL-10,IL-2 and IFN-γ in the supernatants of MLR were examined by ELISA. Results; Compared with DC_(LPS),SV_L,SV_M and SV_H had lower expression of MHC-Ⅱ ,CD40,CD80 and CD86 molecules,secreted less IL-12 p40 in the supernatant,and exhibited stronger antigen phagocytosis capability; moreover, SV_L >SV_M and SV_H showed significantly weaker activity in stimulating proliferation of allogeneic T cells(P<0. 05,0. 01). Compared with DC_(LPS) ,SV_L ,SV_M and SV_H groups had significantly decreased IL-2 and INF-γ levels in MLR supernatants (P<0. 05), but there was no significant difference in the secretion of IL-10. Conclusion; Salvianolate (50-200 μg/ml) can inhibit the maturation of mouse BMDCs in vitro ,and thus has a negative effect on immunologic function of BMCDs.