Effect of Dietary Lipids (Soybean Lecithin and Triacylglycerol) on Hepatic F-Actin Microfilaments in Cyclosporine A-Treated Rats |
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| Authors: | Liliane Benkoel Fran¸coise Chanussot Frank Dodero Caroline de la Maisonneuve Pierre Bongrand Anne Marie Benoliel Renée Lambert Joelle Brisse Albert Chamlian |
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| Affiliation: | (1) Laboratoire de Pathologie Cellulaire et M´etabolique du Foie, INSERM U260, Facult´e de M´edecine, 27 boulevard Jean Moulin, 13385 Marseille Cedex 05;(2) INSERM U 476, 18 avenue Mozart, 13009 Marseille; and;(3) INSERM U 387, H^opital Sainte Marguerite, boulevard Sainte Marguerite, 13009 Marseille, France |
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| Abstract: | We studied and quantified the effect of cyclosporine A on hepatic F-actin on bile canalicular and basolateral membranes in rats fed either soybean lecithin, triacylglycerol-enriched diet, or low-fat diet by means of confocal laser scanning microscopy imaging. The phalloidin–FITC staining of F-actin was quite normal in the lecithin–cyclosporine A group but decreased significantly in the other cyclosporine A-treated groups (by 40% and 25% of control in triacylglycerol–cyclosporine A and cyclosporine A groups, respectively). The alteration of F-actin by cyclosporine A, related to cholestasis evidenced by a decrease in bile salt secretion, was prevented by dietary soybean lecithin and amplified by dietary soybean triacylglycerol. |
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| Keywords: | cyclosporine A dietary lipids cholestasis rat hepatocyte F-actin confocal laser scanning microscopy |
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