梗阻性黄疸后大鼠腹腔巨噬细胞的功能改变

目的:探讨梗阻性黄疸对腹腔巨噬细胞功能的影响。方法:结扎Wistar大鼠胆管,制成梗阻性黄疸模型,30只大鼠随机分为对照组和黄疸组,于梗阻性黄疸10天后,利用双抗夹心酶联免疫吸附法(ELISA)分析腹腔巨噬细胞释放的肿瘤坏死因子(TNF-α)及白细胞介素-1(IL-1)的情况,同时运用流式细胞仪检测和评价两组腹腔巨噬细胞的凋亡及吞噬凋亡细胞能力的变化。结果:(1)黄疸组大鼠腹腔巨噬细胞释放TNF-α101.24±7.9pg/ml、IL-19.7±0.8pg/ml较对照组206.12±10.7pg/ml、31.04±1.7pg/ml显著减少(P<0.01);(2)黄疸组大鼠腹腔巨噬细胞凋亡比例为8.07±0.83%,较对照组1.59±0.41%明显增高(P<0.01);(3)黄疸组腹腔巨噬细胞吞噬凋亡细胞的比例17.49±2.6%,较对照组38.47±6.98%明显降低(P<0.01)。结论:梗阻性黄疸后机体免疫功能降低,腹腔巨噬细胞功能明显受损,细胞凋亡能力增加,是机体易感性增加的重要机制。

Change of Peritoneal Macrophages Function after Obstructive Jaundice

Objective:To investigate the effect of obstructive jaundice(OJ)on the peritoneal macrophage function in rats.Methods:Adult male and female Wistar rats were randomly divided into two groups:control group(n=10)and OJ group(n=20).The model of OJ was created.Ten days after OJ,TNF-α and IL-1 were measured by ELISA in peritoneal macrophage supernatants.Moreover,flow cytometry was employed to detect and evaluate the change of the apoptosis and the phagocytosis capacity in vitro in peritoneal macrophages harvested from the rats.Results:(1)In OJ group,TNF-α(101.24±7.9 pg/ml)and IL-1(9.7±0.8 pg/ml)were significantly lower than thoses in control group,TNF-α(206.12±10.7 pg/ml)and IL-1(31.04±1.7 pg/ml)(P<0.01);(2)Apoptosis proportion of peritoneal macrophages in OJ group(8.07±0.83﹪)was significantly higher than in control group(1.59±0.41﹪)(P<0.01);(3)The phagocytosis capacity in OJ group(17.49±2.6﹪)is lower than that in control group(38.47±6.98﹪)(P<0.01).Conclusion:The result may support that decreased immune function and increased apoptotic cells after OJ appear to be an important factor by which susceptibility is increased,that the function of peritoneal macrophage was impaired after OJ and that the apoptotic peritoneal macrophages are increased.