赤藓糖醇对变异链球菌作用机制的实验研究

目的研究赤藓糖醇对变异链球菌细胞壁结构的影响,探讨其抑制变异链球菌生长的机制。方法测定在蔗糖和赤藓糖醇条件下变异链球菌所在的液体培养基中乳酸脱氢酶（LDH）的活性;在扫描电镜下观察变异链球菌表面的形态变化,了解赤藓糖醇对变异链球菌细胞壁的影响。结果在赤藓糖醇组中,变异链球菌所在的液体培养基内LDH的含量和蔗糖组有差别,但差别甚微。扫描电镜显示：在赤藓糖醇组中,变异链球菌的表面形态清晰,没有内容物溢出的迹象。结论赤藓糖醇可能不是通过影响细胞壁结构和功能的完整性而抑制变异链球菌的。

Study on the mechanism of erythritol effecting on Streptococcus mutans

Objective To evaluate the effect of erythritol on cell wall structure of Streptococcus mutans(S.mutans) and explore its potential mechanism.Methods Enzyme activities of lactate dehydrogenase(LDH) in bacterial solution were detected under respective condition of sucrose and erythritol.A scanning electron microscope(SEM) was used to investigate the change of S.mutans’ cell wall under the condition of sucrose and erythritol.Results Enzyme activities of LDH in erythritol culture medium were different from that in sucrose,but the difference was slight.SEM observation showed the integrity of cell wall was not destroyed and no content leaked out.Conclusion It’s suggested that erythri-tol has an antibacterial effect on S.mutans through no affecting on the normal structure of the cell wall of S.mutans.