含红花血清干预后大鼠肺动脉内皮细胞Ps、ICAM-1基因表达变化及意义

目的观察含红花血清在缺氧复氧条件下对大鼠肺动脉内皮细胞（RPAECs）P-选择素（Ps）及细胞间黏附分子-1（ICAM-1）基因表达的影响，探讨肺栓塞溶栓后缺血再灌注损伤的机制。方法取SPF级雄性大鼠（18只）RPAECs原代培养后分为四组，常氧组持续通入空气12h；缺氧组通入95％N2、5％CO2混合气12h;复氧组在缺氧1h后通95％O2、5％CO2混合气复氧12h；干预组在缺氧再复氧条件下内皮细胞培养液中加含红花血清2ml。观察不同条件下1、6、12h RPAECs中P5、ICAM-1基因表达的变化。结果常氧组各时间点Ps及ICAM-1 mRNA水平无明显变化，缺氧组各时间点Ps及ICAM-1 mRNA水平有所上调，但与常氧组比较无统计学意义。复氧组各时间点Ps及ICAM-1 mRNA及蛋白表达均明显上调；红花干预组各时间点Ps及ICAM-1 mRNA上调水平明显低于复氧组，P〈0．05。结论缺氧复氧条件下Ps及ICAM-1 mRNA过度表达可能参与了肺栓塞溶栓后缺血再灌注损伤的发病；红花注射液可抑制缺氧复氧条件下Ps及ICAM-1 mRNA的过度表达。

Change of P-selectin and ICAM-1 in pulmonary artery endothelium cell on condition of hypoxia and reoxygenation containing safflor serum culture

Objective] To observe the effect of serum containing safflor injection on P-selectin(Ps) and ICAM-1 in pulmonary artery endothelium cell during hypoxia and reoxygenation,and to analyze the mechanism of pulmonary ischemic/reperfusion injury after thrombolytic in pulmonary thromboembolism.Methods] 2 ml/kg safflor was injected from vena caudalis into 10 SPF class male rats to obtain containing safflor serum;Primarily cultured the rat pulmonary artery endothelial cells,and the cells were divided into 4 groups according to different conditions,normoxia group was exposed to atmosphere for 12 hour;hypoxia group was exposed to 95%N_2,5% CO_2 for 12 hours;reoxygenation group was exposed to 95% O_2,5%CO_2 for 12 hours after to hypoxia for 1 hour;safflor intervention group was given containing safflor serum on condition of hypoxia and reoxygenation.Observed the expression of Ps and ICAM-1 at 1,6,12 hour during normoxia,hypoxia and reoxygenation respectively and the effect of serum safflor injection on the expression of Ps and ICAM-1 mRNA after reoxygenation.Result] The expression of Ps and ICAM-1 mRNA in cultured cell in normoxia group had not obvious changes at every time point.They were increased in hypoxia group,but compared with the normoxia group,there was no significant difference.The expression of mRNA and protein of Ps and ICAM-1 were all increased significantly at 1, 6,12 hour after reoxygenation.Adding serum containing safflor after reoxygenation,the expression of Ps,ICAM-1 mRNA were all decreased significantly at each time point,compared with normoxia and reoxygenation group,there were obvious differences(P<0.05).Conclusions] The overexpression of Ps and ICAM-1 during reoxygenation may participate in the pathogenesis of ischemic/reperfusion injury after thromblytic in pulmonary thromboembolism.Safflor injection may relieve the ischemic/reperfusion injury.