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AM真菌对滇重楼光合参数及生理指标的影响
引用本文:韦正鑫,郭冬琴,李海峰,丁博,张杰,周浓,于杰. AM真菌对滇重楼光合参数及生理指标的影响[J]. 中国中药杂志, 2015, 40(20): 3945-3952
作者姓名:韦正鑫  郭冬琴  李海峰  丁博  张杰  周浓  于杰
作者单位:西 南 大学园艺园林学院, 重庆 400716;大理大学药学与化学学院, 云南 大理 671000,重庆三峡学院生命科学与工程学院, 重庆 404000,大理大学药学与化学学院, 云南 大理 671000,重庆三峡学院生命科学与工程学院, 重庆 404000,重庆三峡学院生命科学与工程学院, 重庆 404000,大理大学药学与化学学院, 云南 大理 671000;重庆三峡学院生命科学与工程学院, 重庆 404000,西 南 大学园艺园林学院, 重庆 400716
基金项目:国家自然科学基金项目(81260622);云南省应用基础研究计划项目(2011FB081);云南省教育厅科学研究基金重点项目(2012Z119)
摘    要:通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对滇重楼光合参数和生理生化指标的影响。结果表明,滇重楼与AM真菌形成了良好的共生关系。AM真菌诱导处理后,不同AM真菌对滇重楼叶片的光合参数和生理生化指标的影响有所差异,AM真菌的施用能够提高滇重楼叶肉细胞的光合作用强度,提高滇重楼叶片可溶性蛋白和可溶性糖含量及保护酶活性,增强了滇重楼抗不利环境的能力,促进了滇重楼的生长,而滇重楼与AM真菌之间存在一定的相互选择性。从综合接种效应来看,瑚状盾巨囊霉Racocetra coralloidea、美丽盾巨孢囊霉Scutellospora calospora、近明球囊霉Claroideoglomus claroideum、透明盾巨孢囊霉S. pellucida和明球囊霉Rhizophagus clarus是最适宜滇重楼大田栽培时推广应用的优良备选菌株。

关 键 词:滇重楼  AM真菌  光合特性  生理生化指标  抗逆性
收稿时间:2015-04-23

Photosynthetic parameters and physiological indexes of Paris polyphylla var. yunnanensis influenced by arbuscular mycorrhizal fungi
WEI Zheng-xin,GUO Dong-qin,LI Hai-feng,DING Bo,ZHANG Jie,ZHOU Nong and YU Jie. Photosynthetic parameters and physiological indexes of Paris polyphylla var. yunnanensis influenced by arbuscular mycorrhizal fungi[J]. China Journal of Chinese Materia Medica, 2015, 40(20): 3945-3952
Authors:WEI Zheng-xin  GUO Dong-qin  LI Hai-feng  DING Bo  ZHANG Jie  ZHOU Nong  YU Jie
Affiliation:College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China;College of Pharmacy and Chemistry, Dali University, Dali 671000, China,College of Life Science & Engineering, Chongqing Three Gorges University, Chongqing 404000, China,College of Pharmacy and Chemistry, Dali University, Dali 671000, China,College of Life Science & Engineering, Chongqing Three Gorges University, Chongqing 404000, China,College of Life Science & Engineering, Chongqing Three Gorges University, Chongqing 404000, China,College of Pharmacy and Chemistry, Dali University, Dali 671000, China;College of Life Science & Engineering, Chongqing Three Gorges University, Chongqing 404000, China and College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China
Abstract:Through potted inoculation test at room temperature and indoor analysis, the photosynthetic parameters and physiological and biochemical indexes of Paris polyphylla var. yunnanensis were observed after 28 arbuscular mycorrhizal(AM) fungi were injected into the P. polyphylla var. yunnanensis growing in a sterile soil environment. The results showed that AM fungi established a good symbiosis with P. polyphylla var. yunnanensis. The AM fungi influenced the photosynthetic parameters and physiological and biochemical indexes of P. polyphylla var. yunnanensis. And the influences were varied depending on different AM fungi. The application of AM fungi improved photosynthesis intensity of P. polyphylla var. yunnanensis mesophyll cells, the contents of soluble protein and soluble sugar, protective enzyme activity of P. polyphylla var. yunnanensis leaf, which was beneficial to resist the adverse environment and promote the growth of P. polyphylla var. yunnanensis. Otherwise, there was a certain mutual selectivity between P. polyphylla var. yunnanensis and AM fungi. From the comprehensive effect of inoculation, Racocetra coralloidea, Scutellospora calospora, Claroideoglomus claroideum, S. pellucida and Rhizophagus clarus were the most suitable AM fungi to P. polyphylla var. yunnanensis when P. polyphylla var. yunnanensis was planted in the field.
Keywords:Paris polyphyllavar.yunnanensis  AM fungi  the photosynthetic parameters  physiological and biochemical indexes  resistance
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