| 人工诱导海南龙血树所产血竭的抗菌活性成分研究 |
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| 引用本文： | 蒋和梅,王辉,王军,戴好富,骆焱平,梅文莉. 人工诱导海南龙血树所产血竭的抗菌活性成分研究[J]. 中国中药杂志, 2015, 40(20): 4002-4006 |
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| 作者姓名： | 蒋和梅 王辉 王军 戴好富 骆焱平 梅文莉 |
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| 作者单位： | 海南 大学环境与植物保护学院, 海南 海口 570228;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室, 海南 海口 571101,中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室, 海南 海口 571101,中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室, 海南 海口 571101,中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室, 海南 海口 571101,海南 大学环境与植物保护学院, 海南 海口 570228,中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室, 海南 海口 571101 |
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| 基金项目： | 国家自然科学基金项目(31070321,31400297);海南省中药现代化专项(2015ZY17);海南省重大科技项目(ZDZX2013008-4) |
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| 摘 要： | 为了研究人工诱导海南龙血树Dracaena cambodiana所产血竭的化学成分,该研究采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱对其进行分离纯化,并运用波谱方法对所分离的化合物进行结构鉴定。研究从人工诱导海南龙血树所产血竭的乙醇提取物中共分离鉴定出10个化合物,经波谱解析鉴定为3,4-二羟基烯丙基苯(1)、3',4',5'-三甲氧基苯丙烯醇(2)、松脂醇(3)、(2R)-7, 4'-二羟基-8-甲基黄烷(4)、(2R)-7, 4'-二羟基-5-甲氧基-8-甲基黄烷(5)、(2S)-7, 3'-二羟基-4'-甲氧基-8-甲基黄烷(6)、(2S)-4', 7-二羟基-6,8-二甲基黄烷(7)、4, 2', 4'-三羟基查耳酮(8)、4, 4'-二羟基-2-甲氧基二氢查耳酮(9)和Cambodianin E(10)。抗菌活性测试结果表明化合物1,4,10对香蕉枯萎菌Fusarium oxysporum f. sp. cuben,西瓜枯萎菌F. oxysporum f. sp. niveum,棉花枯萎菌F. oxysporum f. sp. vasinfectum和烟草青枯菌Ralstonia solanacearum有抑制作用。
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| 关 键 词： | 海南龙血树 人工诱导血竭 化学成分 抗菌活性 |
| 收稿时间： | 2015-05-22 |
Antibacterial components from artificially induced dragon's blood of Dracaena cambodiana |
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| JIANG He-mei,WANG Hui,WANG Jun,DAI Hao-fu,LUO Yan-ping and MEI Wen-li. Antibacterial components from artificially induced dragon's blood of Dracaena cambodiana[J]. China Journal of Chinese Materia Medica, 2015, 40(20): 4002-4006 |
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| Authors: | JIANG He-mei WANG Hui WANG Jun DAI Hao-fu LUO Yan-ping MEI Wen-li |
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| Affiliation: | College of Environment and Plant Protection, Hainan University, Haikou 570228, China;Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China,Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China,Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China,Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China,College of Environment and Plant Protection, Hainan University, Haikou 570228, China and Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China |
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| Abstract: | Ten compounds were isolated from the artificially induced dragon's blood of Dracaena cambodiana by various column chromatographies on silica and sephadex LH-20 gel. Based on spectral analysis of NMR and MS, their structures were identified as 3,4-dihydroxyallylbenzene(1), 3',4',5'-trimethoxycinnamylalcohol(2), pinoresinol(3),(2R)-7,4'-dihydroxy-8-methylflavane(4),(2R)-7,4'-dihydroxy-5-methoxy-8-methylflavane(5),(2S)-7,3'-dihydroxy-4'-methoxy-8-methylflavane(6),(2S)-4',7-dihydroxy-6,8-dimethylflavane(7), 4,2',4'-trihydroxychalcone(8), 4,4'-dihydroxy-2-methoxydihydrochalcon(9) and Cambodianin E(10). Antibacterial activity assay showed that compounds 1, 4 and 10 have inhibitory effect on Fusarium oxysporum f. sp. cuben, Fusarium oxysporum f. sp. niveum, Fusarium oxysporum f. sp. vasinfectum and Ralstonia solanacearum. |
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| Keywords: | Dracaena cambodiana artificially induced Dragon's blood chemical constituents antibacterial activities |
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