p53,c-myc,nm23癌基因蛋白在喉复发癌的表达及其意义

目的：研究p53,c-myc,nm23癌基因蛋白的表达与喉癌复发的关系，对预后作出估计。方法：回顾分析喉癌手术标本574例，术后随访3年，确诊术后复发56例。正常喉对照标本39例。标本制成组织芯片，采用免疫组化SABC法对芯片标本进行染色。结果：p53,nm23、c-myc在喉癌组织中有表达，其阳性率各有不同，其中c-myc在喉癌复发组的表达明显增强，与未复发组的表达差异有极显著性意义（P＜0．01）；而nm23在喉癌复发组的表达明显减弱，与未复发组的表达差异有极显著性意义（P＜0．01）。p53在喉癌复发组与复发组的表达差异无显著性意义（P＞0．05）。c-myc的表达与喉癌复呈正相关，nm23癌基因蛋白的表达与喉癌复发呈负相关。结论：c-myc,nm23癌基因蛋白的表达可作为预测喉癌术后复发的指标。

Significance of p53, c-myc and nm23 protein expression in recurrent laryngeal carcinoma

Objective:To evaluate the prognosis of postoperative laryngeal cancer recurrence, the study was designed to investigate the relationship of p53, c-myc and nm23 protein expression in recurrent laryngeal carcinoma.Method:Five hundred and seventy-four cases of laryngeal carcinoma were retrospectively reviewed. All the patients were observed for 3 years postoperatively, 56 cases with recurrence were diagnosed and 39 cases of normal laryngeal tissue were compared.The tissue microarrays was made in all the cases. The tissue chip was analyzed by the SABC immunohistochemical method.Result:p53, c-myc and nm23 expressed in most of the laryngeal carcinoma. The positive ratio is different. There is higher expression of c-myc in recurrent laryngeal carcinoma, and its expression has significant difference with the laryngeal carcinoma, which have no recurrence (P< 0.01). nm23 has lower expression in recurrent laryngeal carcinoma , and it's expression has significant difference with the laryngeal carcinoma which have no recurrence(P< 0.01).The expression of p53 has no significant difference between recurrent laryngeal carcinoma and the laryngeal carcinoma which have no recurrence(P> 0.05).There is the positive correlation between the expression of c-myc and the recurrent laryngeal carcinoma; and negative correlation between the expression of nm23 and the recurrent laryngeal carcinoma.Conclusion:c-myc and nm23 could be the prognosis marker of postoperative laryngeal cancer recurrence.