| 补骨脂素逆转人乳腺癌细胞多药耐药性的研究 |
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| 引用本文： | 蔡宇. 补骨脂素逆转人乳腺癌细胞多药耐药性的研究[J]. 肿瘤, 2004, 24(3): 240-241 |
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| 作者姓名： | 蔡宇 |
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| 作者单位： | 暨南大学药学院,广州,510632 |
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| 基金项目： | 国家自然科学基金，国家中医药管理局科研项目，浙江省教育厅资助项目 |
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| 摘 要： | 目的研究中药补骨脂素对人乳腺癌细胞多耐药性的逆转作用.方法用MTT法测定药物的细胞毒性和IC50用流式细胞仪测定耐药细胞P170糖蛋白表达,并选异搏定作阳性对照,观察具有钙拮抗作用的补骨脂素对MCF-7/ADR多药耐药性的逆转作用.结果补骨脂素在非细胞毒性剂量下能使MCF-7/ADR对阿霉素的浓度升高,但对细胞表面的糖蛋白P-170却没有影响.结论补骨脂素具有逆转人乳腺癌MCF-7/ADR多药耐药性的作用.
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| 关 键 词： | 乳腺肿瘤 MCF-7细胞 MCF-7/ADR细胞 补骨脂素类 多药耐药抗药 |
| 文章编号： | 1000-7431(2004)03-0240-02 |
| 修稿时间： | 2003-04-02 |
Reversal of multidrug resistance in human breast cancer cell by psoralen |
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| CAI Yu .. Reversal of multidrug resistance in human breast cancer cell by psoralen[J]. Tumor, 2004, 24(3): 240-241 |
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| Authors: | CAI Yu . |
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| Abstract: | Objective To investigate the effect of reversing multidrug resistance (MDR) of psoralen in human breast cancer cell. Methods Psoralen, one kind of herd medicine, was selected and its reversal effect to MCF 7/ADR cell line was studied. MTT method used to determine the toxic effect and IC 50 and was used to examine the expression of P170 protein of multidrug resistance cell. Verapamil was used as positive control.Results Psoralen could reduce the value of IC 50 of adriamycin against MDR cells line MCF 7/ADR at non cytotoxic concentration and enhance the accumulation of adriamycin,but no marked change in expression of P 170 protein was observed. Conclusion Psoralen can reverse MDR of MCF 7/ADR cell of human breast cancer. |
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| Keywords: | Breast neoplasms MCF-7 cells MCF-7/ADR cells Psoralens resistance Multidrug resistance |
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