应用逆转录病毒pLXSN-GFP在脑组织追踪骨髓源干细胞的可行性

目的 探讨应川逆转录病毒pLXSN-GFP在脑组织追踪骨髓源干细胞的可行性。方法 应用逆转录病毒pLXSN-GFP标记Sprague-Dawley大鼠骨髓源干细胞，经尾静脉植入C57BL/6小鼠。在植入后4周和8周取材。用荧光显微镜和激光共聚焦显微镜观察绿色荧光阳性细胞．同时用GFP抗体的荧光免疫组织化学方法判断绿色荧光的来源。结果移植骨髓源干细胞后4周和8周，在嗅球和脑干网状结构均町见绿色荧光阳性细胞，GFP抗体的荧光免疫组织化学方法证实绿色荧光的确来源于GFP的表达。结论 逆转录病毒pLXSN-GFP可用以标记并在脑组织追踪骨髓源干细胞。

Feasibility of tracing the bone marrow-derived stem cells in brain tissue with retrovirus pLXSN-GFP

Objective To explore the feasibility of tracing the bone marrow-derived stem cells in brain tissue with retrovirus pLXSN-GFP. Methods The bone marrow-derived stem cells (BMDSCs) from male Sprague-Dawley rat were labeled with retrovirus pLXSN-GFP and then transplanted into the female C57BL/6 mice. The mice were sacrificed 4 weeks and 8 weeks after the transplantation of GFP-labeled BMDSCs. Fluorescence microscope and confocal laser scanning microscope showed positive cells with green fluorescence whose source were searched for by means of fluorescence immunohistochemistry of GFP antibody. Results The green fluorescence positive cells were found in olfactory bulb and brainstem reticular formation 4 weeks and 8 weeks after the transplantation of GFP-labeled BMDSCs. Fluorescence immunohistochemistry of GFP antibody demonstrated the green fluorescence did come from the expression of GFP. Conclusion It is possible to trace the bone marrow-derived stem cells in brain tissue with retrovirus pLXSN-GFP.