|
|||||
|
|
Size-dependent tissue kinetics of PEG-coated gold nanoparticles |
|
| Authors: | Wan-Seob Cho Minjung Cho Mina Choi Hyung-Seon Shin Bong Hyun Chung Myung-Haing Cho |
| Affiliation: | a ELEGI/Colt Laboratory, Centre for Inflammation Research, The Queen's Medical Research Institute, The University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK b Department of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration, Seoul 122-704, Republic of Korea c Nanobiotechnology, School of Engineering, Korea University of Science and Technology, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea d Division of Earth and Environmental Science, Environmental Tracer Team, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea e Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration, Seoul 122-704, Republic of Korea f Laboratory of Toxicology, College of Veterinary Medicine, and Department of Nanofusion Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea |
| Abstract: | Gold nanoparticles (AuNPs) can be used in various biomedical applications, however, very little is known about their size-dependent in vivo kinetics. Here, we performed a kinetic study in mice with different sizes of PEG-coated AuNPs. Small AuNPs (4 or 13 nm) showed high levels in blood for 24 h and were cleared by 7 days, whereas large (100 nm) AuNPs were completely cleared by 24 h. All AuNPs in blood re-increased at 3 months, which correlated with organ levels. Levels of small AuNPs were peaked at 7 days in the liver and spleen and at 1 month in the mesenteric lymph node, and remained high until 6 months, with slow elimination. In contrast, large AuNPs were taken up rapidly (∼ 30 min) into the liver, spleen, and mesenteric lymph nodes with less elimination phase. TEM showed that AuNPs were entrapped in cytoplasmic vesicles and lysosomes of Kupffer cells and macrophages of spleen and mesenteric lymph node. Small AuNPs transiently activated CYP1A1 and 2B, phase I metabolic enzymes, in liver tissues from 24 h to 7 days, which mirrored with elevated gold levels in the liver. Large AuNPs did not affect the metabolic enzymes. Thus, propensity to accumulate in the reticuloendothelial organs and activation of phase I metabolic enzymes, suggest that extensive further studies are needed for practical in vivo applications. |
| Keywords: | Size-dependent kinetics Metabolism Gold nanoparticles Mouse |
| 本文献已被 ScienceDirect 等数据库收录！ | |