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Experimental Model of Isolated Lung Perfusion in Rats: First Brazilian Experience Using the IL-2 Isolated Perfused Rat or Guinea Pig Lung System |
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| Authors: | P.M. Pê go-Fernandes,E. Werebe,R. Pazetti,P.R.O. Soares |
| Affiliation: | a Department of Cardio-Pulmonology, Heart Institute (InCor), Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil b Thoracic Surgery Research Laboratory, Heart Institute (InCor), Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil c Post Graduate Program, Division of Thoracic Surgery, Heart Institute (InCor), Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil d Department of Surgery, Division of Thoracic Surgery, Federal University of Medical Sciences of Porto Alegre (UFCSPA), Porto Alegre, Rio Grande do Sul-Brazil |
| Abstract: | IntroductionLung transplantation has become the mainstay therapy for patients with end-stage lung disease refractory to medical management. However, the number of patients listed for lung transplantation largely exceeds available donors. The study of lung preservation requires accurate, cost-effective small animal models. We have described a model of ex vivo rat lung perfusion using a commercially available system.MethodsMale Wistar rats weighing 250 g-300 g were anesthetized with intraperitoneal sodium thiopental (50 mg/kg body weight). The surgical technique included heart-lung block extraction, assembly, and preparation for perfusion and data collection. We used an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus, Holliston, Mass, United States; Hugo Sachs Elektronik, Alemanha).ResultsPreliminary results included hemodynamic and pulmonary mechanics data gathered in the experiments.ConclusionThe isolated rat lung perfusion system is a reliable method to assess lung preservation. |
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