转铁蛋白增强脂质体介导基因对肝癌细胞的转染和表达

目的：检测转铁蛋白能否增强脂质体介导外源基因对肝癌细胞的转染和表达。方法：应用本实验室构建的含小鼠白细胞介素（mIL)-12的真核表达质粒pcDNA3/mIL-12和绿色荧光蛋白（GFP）真核表达质粒pcDNA3/EGFP,观察转铁蛋白增强阳离子脂质体LipofectAMINE介导上述两种质粒DNA对小鼠和不同人肝癌细胞株的转染及表达效率。结果：LipofectAMINE加转铁蛋白介导pcDNA3/EGFP对小鼠肝癌细胞株Hep1-6的转染效率由2％－5％提高至20％，对4种不同人肝癌细胞株的转染效率由15％提高到65％－75％；但对小鼠肝癌细胞株MM45T.Li的转染效率并不增加。转铁蛋白可使LipofectAMINE介导pcDNA3/mIL-12在Hep1-6中的表达量增强18倍。结论：转铁蛋白能够与LipofectAMINE和质粒DNA形成复合物，显著增强脂质体介导外源基因对肝癌细胞的转染和表达，但对不同种肝癌细胞株的增强效率不同。

Enhancement of Liposome-mediated Gene Transfection and Expression in HepatomaCells by Transferrin

Background Aims: To detect the enhancement of liposome-mediated gene transfection and ex- pression in hepatoma cells by transferrin. Methods: Eukaryotic expressing vector containing murine interleukin (mIL)-12 and green fluorescent protein (GFP), i.e. pcDNA3 mIL-12 and pcDNA3 EGFP con- structed in our laboratory were used to detect the enhancement of lipofectAMINE-mediated gene transfection and expression in different hepatoma cell lines of murine and human by transferrin. Re- sults: The transfection rate of murine hepatoma cell line Hepl-6 increased from 2%~5% to 20% by transferrin-lipofectAMlNE-pcDNA3 EGFP complex, and from 15% to 65%~75% in four human hepato- ma cell lines, but did not increase in murine hepatoma cell line MM45T.Li. Transferrin increased mIL- 12 expression in Hepl-6 by lipofcctAMlNE-mediated pcDNA3 mIL-12 about 18 folds over lipofect- AMINE transfection alone. Conclusions: The transferrin-lipofectAMlNE-plasmid DNA complex can be formed. Transferrin can increase the transfection and expression rates of foreign gene in hepatoma cells significantly as mediated by lipofectAMlNE. but the enhancement rates of different hepatoma cell lines are different.