Twist介导的上皮间质转化在卵巢癌组织中的作用

  目的 检测卵巢癌组织中Twist、E-cadherin及N-cadherin蛋白和mRNA的表达，探讨Twist介导的上皮间质转化（EMT）现象在卵巢癌发生、发展及转移中的作用。方法 分别采用免疫组化方法和RT-PCR法检测42例卵巢癌组织中介导EMT发生的转录因子Twist、E-cadherin及N-cadherin蛋白及其mRNA的表达，30例卵巢良性肿瘤组织作为对照。结果 卵巢癌组织中Twist、E-cadherin及N-cadherin蛋白阳性表达率分别为66.7%、28.6%和47.6%，卵巢良性肿瘤组织分别为26.7%、90.0%和13.3%,;Twist、E-cadherin及N-cadherin mRNA在卵巢癌组织中的表达量分别为(1.49±0.53)、(0.82±0.24)、(1.55±0.56)，卵巢良性肿瘤组织中分别为(1.18±0.34)、(1.09±0.20)、(1.18±0.35)。两种组织各项指标比较差异有统计学意义（P<0.05）。Twist、E-cadherin及N-cadherin的表达与卵巢癌临床分期、分化程度及淋巴结转移有关（P <0.05）。结论 在卵巢癌组织中，Twist、N-cadherin表达上调，E-cadherin表达下调，Twist介导的EMT可能与卵巢癌的侵袭转移有关。

Effect of Twist on Epithelial-mesenchymal Transitions in Ovarian Cancer Tissue

Objective To observe the expression of Twist,E-cadherin and N-cadherin in ovarian cancer tissue and investigate the role of epithelial-mesenchymal transitions(EMT) in metastasis of ovarian cancer.Methods The expression of Twist,E-cadherin and N-cadherin proteins and their mRNA in 42 ovarian cancer cases was detected by immunohistochemistry and RT-PCR,and 30 cases of ovarian benign tumor were selected as control group.Results The positive expression rates of Twist,E-cadherin and N-cadherin proteins in ovarian cancer tissue were 66.7%,28.6% and 47.6% respectively,whereas in the control group the positive expression rates were 26.7%,90.0% and 13.3%;The expression levels of Twist,E-cadherin and N-cadherin mRNA were 1.49±0.53,0.82±0.24 and 1.55±0.56,whereas in the control group the expression levels were 1.18±0.34,1.09±0.20 and 1.18±0.35.There was statistical difference between the two groups(P <0.05).Conclusion The expression levels of Twist and N-cadherin are upregulated,and the expression level of E-cadherin is downregulated.The role of Twist in EMT-mediated invasiveness may be related to metastasis and progression of ovarian cancer.