胰岛素抵抗大鼠脂肪组织chemerin mRNA的表达和意义

 目的 探讨胰岛素抵抗大鼠脂肪组织中脂肪因子chemerin mRNA的表达情况，探讨其与胰岛素抵抗之间的关系及意义。 方法 采用高脂饲料喂养建立胰岛素抵抗大鼠模型，将30只雄性SD大鼠随机分为正常饮食组（NC，n=15）和高脂饮食组（HF，n=15），喂养10周后，以大鼠清醒状态下正常血糖-高血浆胰岛素钳夹技术进行评估，处死后取肾周脂肪组织，应用Real-time PCR方法检测大鼠脂肪组织中chemerin mRNA的表达水平。结果 (1)与NC组比较，HF组60～120 min的平均葡萄糖输注率（GIR60～120）明显降低(13.87±1.45) mg·kg-1·min-1 vs (24.10±2.87) mg·kg-1·min-1，P<0.05]。(2)与NC组比较，HF组大鼠脂肪组织中chemerin mRNA的表达明显升高(1.86±1.02) vs (0.92±0.32)，P<0.01]。结论 胰岛素抵抗大鼠脂肪组织chemerin mRNA升高，提示脂肪因子chemerin可能参与胰岛素抵抗的发生。

Expressions and Significance of chemerin mRNA in Adipose Tissue of Insulin Resistant Rats

Objective To investigate the expressions of chemerin mRNA in adipose tissue of insulin resistant rats and the relationship between them.Methods Insulin resistant rats were induced by high-fat diet feeding.Thirty male SD rats were randomly divided into two groups fed with either basic chow(NC,n =15) or high-fat diet(HF,n =15) for 10 weeks,then assessed by euglycemic-hyperinsulinemia clamp technique.Rats were sacrificed and taken the perinephrit adipose tissue.The chemerin mRNA expression in adipose tissue was tested by real-time PCR.Results(1) The glucose infusion rate(GIR60-120) was significantly decreased in HF group compared with control group,GIR60-120(13.87±1.45 mg·kg-1·min-1 vs 24.10±2.87 mg·kg-1·min-1,P < 0.05).(2)The chemerin mRNA expression in adipose tissue was significantly increased in HF group compared with control group(1.86±1.02 vs 0.92±0.32,P < 0.01).Conclusion The increased expression of chemerin mRNA in adipose tissue of insulin resistant rats showed that chemerin might be involved in insulin resistance.