猪心肌梗死后室性心律失常模型建立方法的比较

目的比较不同的急性心肌梗死(简称心梗)造模方法导致室性心律失常发生的差异,探索最佳造模方法。方法 45只苏中幼猪随机分为假手术组(Sham组,n=5)、开胸结扎左前降支(LAD)组(结扎组,n=17)和经导管球囊封堵LAD组(封堵组,n=23)。结扎/封堵后90 min内心电图监测动物室性心律失常发生情况。饲养8周后,以2,3,5-氯化三苯基四氮唑(TTC)染色、HE染色鉴定梗死模型是否成功。结果与结扎组相比,封堵组术后即刻存活率低(60.87%vs 94.12%,P<0.05),室性早搏、室性心动过速的发生率没有明显差异(91.30%vs 70.59%,30.43%vs 29.41%;P均>0.05);但封堵组心室颤动发生率明显增高(69.57%vs 5.88%,P<0.001)。8周后经TTC、HE染色鉴定,两组造模成功率无显著差异(68.75%vs 92.86%,P=0.234),但是封堵组梗死面积较大。结论与结扎法相比,封堵法可以导致大面积心梗,室性心律失常发生率高。

The occurrences of ventricular arrhythmia after acute myocardial infarction in a porcine model: comparison between ligation and occlusion

Objective To compare the differences of ventricular arrhythmia occurrences among different models of acute myocardial infarction(MI).Methods Forty-five pigs were randomly divided into 3 groups: Sham operation group(Sham group,n=5),left anterior descending(LAD) artery ligation-induced MI model(ligation group,n=17) and balloon catheter based LAD occlusion-induced MI model(occlusion group,n=23).The occurrences of ventricular arrhythmias were recorded by ECG monitoring after 90 minutes of ligation or occlusion.8 weeks later,2,3,5-triphenyl tetrazolium chloride(TTC) staining,HE staining were used to examine whether the infarction model was successful.Results The immediate survival rate of occlusion group was lower(60.87% vs 94.12%,P=0.042).There were no differences between the two different MI models in regards to the incidence of premature ventricular contractions and ventricular tachycardia(91.30% vs 70.59%,30.43% vs 29.41%;both P>0.05) during the operation procedure,but the incidence of ventricular fibrillation was significantly higher in occlusion group(69.57% vs 5.88%,P<0.001).After 8 weeks,TTC and HE staining showed that there was no significant difference in the model success rate(68.75% vs 92.86%,P = 0.234),but the infarct area was higher in the occlusion group.Conclusions Compared with conventional ligation methods,LAD occlusion methods can lead to a large area of MI and the higher incidence of ventricular arrhythmias.