| 藻毒素免疫抗原的制备及鉴定 |
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| 引用本文： | 赵晓联,孙蔚榕,孙秀兰,汤坚. 藻毒素免疫抗原的制备及鉴定[J]. 卫生研究, 2005, 34(6): 726-729 |
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| 作者姓名： | 赵晓联 孙蔚榕 孙秀兰 汤坚 |
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| 作者单位： | 1. 江苏省微生物研究所,无锡,214063
2. 江南大学 |
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| 基金项目： | 国家“十五”科技专项(No.2001BA804A20),江苏省自然基金(No.BK2002406) |
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| 摘 要： | 目的为建立藻毒素(MCLR)免疫学检测方法,制备具免疫功能的MCLR完全抗原。方法根据MCLR分子中的羧基和蛋白质分子中的氨基,利用“活泼酯法”和“碳二亚胺法”分别制备MCLRKLH(匙孔血蓝蛋白)免疫抗原和MCLRBSA(牛血清白蛋白)检测抗原。结果用紫外光谱法(UV)和Bradford蛋白含量测定法鉴定,结合物中MCLR与KLH、BSA的结合摩尔比为9.1∶1和9.4∶1。采用“皮下注射法”用MCLRKLH免疫新西兰大白兔15周,取血清,用“间接酶联免疫吸附法”(id-ELISA)鉴定抗MCLR多克隆抗体效价,最高达1∶25600。结论证明制备的MCLRKLH具有免疫性。
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| 关 键 词： | 藻毒素 免疫抗原 制备 |
| 文章编号： | 1000-8020（2005）06-0726-04 |
| 收稿时间： | 2005-01-14 |
| 修稿时间： | 2005-01-14 |
Preparation and characterization of immunogen against microcystin LR |
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| Zhao Xiao-lian,Sun Wei-rong,Sun Xiu-lan,Tang Jian. Preparation and characterization of immunogen against microcystin LR[J]. Journal of hygiene research, 2005, 34(6): 726-729 |
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| Authors: | Zhao Xiao-lian Sun Wei-rong Sun Xiu-lan Tang Jian |
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| Affiliation: | Jiangsu Institute of Microbiology, Wuxi 214063, China |
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| Abstract: | OBJECTIVE: To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR. METHODS: MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method. RESULTS: The results are 9.1 and 9.4. New Zealand rabbits was immunized by MC-LR-KLH. The titer of the polyantibodies was over 25,600 measured by id ELISA. CONCLUSION: MC-LR-KLH was proved the suitable immunogen against MC-LR. |
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| Keywords: | microcystins preparation immunogen |
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