Presence of synaptonemal complex protein 1 transversal filament-like protein in human primary spermatocytes |
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Authors: | Pousette A; Leijonhufvud P; Arver S; Kvist U; Pelttari J; Hoog C |
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Institution: | Department of Clinical Chemistry, Karolinska Institute, Huddinge University Hospital, Sweden. |
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Abstract: | The synaptonemal complex (SC) is involved in the pairing of chromosomes
during meiosis. We found that antibodies raised against a protein component
(P1) of the mouse synaptonemal complex, mouse SCP1, also identified the SC
in human primary spermatocytes. Biopsies from 18 men presented with
infertility were evaluated by light-field microscopy and grouped into five
categories: normal spermatogenesis, Sertoli cell-only syndrome, meiotic
disturbances, spermiogenic (i.e. differentiation) disturbances, and other
combined disturbances. In all the normal subjects the SCP1 antibody
distinctly stained the synaptonemal complexes of primary spermatocytes,
whereas Sertoli cells, spermatogonia or spermatids were never stained. In
three of the groups, which had germ cells but showed spermatogenic
disturbances, the staining was similar to that seen in normal subjects. In
sharp contrast to this, in sections from men with Sertoli cell-only
syndrome no specific staining was seen. This study demonstrates that a
SCP1-related protein is also conserved in the synaptonemal complex in
meiotic cells from man. Further studies will reveal to what extent the
absence or the non-functionality of SCP1 contributes to male infertility.
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